Bioactive Potential of Marine Macroalgae from the Central Red Sea (Saudi Arabia) Assessed by High-Throughput Imaging-Based Phenotypic Profiling

Kremb, Stephan; Müller, Constanze; Schmitt-Kopplin, Philippe; Voolstra, Christian R.

Bioactive Potential of Marine Macroalgae from the Central Red Sea (Saudi Arabia) Assessed by High-Throughput Imaging-Based Phenotypic Profiling

Dateien

Kremb_2-97yg5189diko8.pdfGröße: 1020.31 KBDownloads: 270

Datum

2017

Autor:innen

Kremb, Stephan

Müller, Constanze

Schmitt-Kopplin, Philippe

Voolstra, Christian R.

Open Access-Veröffentlichung

Open Access Gold

Sammlungen

Biologie

Publikationstyp

Zeitschriftenartikel

Publikationsstatus

Published

Erschienen in

Marine Drugs. MDPI. 2017, 15(3), 80. eISSN 1660-3397. Available under: doi: 10.3390/md15030080

Zusammenfassung

Marine algae represent an important source of novel natural products. While their bioactive potential has been studied to some extent, limited information is available on marine algae from the Red Sea. This study aimed at the broad discovery of new bioactivities from a collection of twelve macroalgal species from the Central Red Sea. We used imaging-based High-Content Screening (HCS) with a diverse spectrum of cellular markers for detailed cytological profiling of fractionated algal extracts. The cytological profiles for 3 out of 60 algal fractions clustered closely to reference inhibitors and showed strong inhibitory activities on the HIV-1 reverse transcriptase in a single-enzyme biochemical assay, validating the suggested biological target. Subsequent chemical profiling of the active fractions of two brown algal species by ultra-high resolution mass spectrometry (FT-ICR-MS) revealed possible candidate molecules. A database query of these molecules led us to groups of compounds with structural similarities, which are suggested to be responsible for the observed activity. Our work demonstrates the versatility and power of cytological profiling for the bioprospecting of unknown biological resources and highlights Red Sea algae as a source of bioactives that may serve as a starting point for further studies.

Fachgebiet (DDC)

570 Biowissenschaften, Biologie

Schlagwörter

bioprospecting; natural products; algae; cytological profiling; High-Content Screening; HIV

Zitieren

ISO 690

KREMB, Stephan, Constanze MÜLLER, Philippe SCHMITT-KOPPLIN, Christian R. VOOLSTRA, 2017. Bioactive Potential of Marine Macroalgae from the Central Red Sea (Saudi Arabia) Assessed by High-Throughput Imaging-Based Phenotypic Profiling. In: Marine Drugs. MDPI. 2017, 15(3), 80. eISSN 1660-3397. Available under: doi: 10.3390/md15030080

BibTex

@article{Kremb2017-03-20Bioac-50745,
  year={2017},
  doi={10.3390/md15030080},
  title={Bioactive Potential of Marine Macroalgae from the Central Red Sea (Saudi Arabia) Assessed by High-Throughput Imaging-Based Phenotypic Profiling},
  number={3},
  volume={15},
  journal={Marine Drugs},
  author={Kremb, Stephan and Müller, Constanze and Schmitt-Kopplin, Philippe and Voolstra, Christian R.},
  note={Article Number: 80}
}

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