Enzymes involved in the anaerobic degradation of ortho-phthalate by the nitrate-reducing bacterium Azoarcus sp. strain PA01

Junghare, Madan; Spiteller, Dieter; Schink, Bernhard

Enzymes involved in the anaerobic degradation of ortho-phthalate by the nitrate-reducing bacterium Azoarcus sp. strain PA01

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Datum

2016

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Open Access Green

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Biologie

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Environmental Microbiology. 2016, 18(9), pp. 3175-3188. ISSN 1462-2912. eISSN 1462-2920. Available under: doi: 10.1111/1462-2920.13447

Zusammenfassung

The pathway of anaerobic degradation of o-phthalate was studied in the nitrate-reducing bacterium Azoarcus sp. strain PA01. Differential two-dimensional protein gel profiling allowed the identification of specifically induced proteins in o-phthalate-grown compared to benzoate-grown cells. The genes encoding o-phthalate-induced proteins were found in a 9.9 kb gene cluster in the genome of Azoarcus sp. strain PA01. The o-phthalate-induced gene cluster codes for proteins homologous to a dicarboxylic acid transporter, putative CoA-transferases and a UbiD-like decarboxylase that were assigned to be specifically involved in the initial steps of anaerobic o-phthalate degradation. We propose that o-phthalate is first activated to o-phthalyl-CoA by a putative succinyl-CoA-dependent succinyl-CoA:o-phthalate CoA-transferase, and o-phthalyl-CoA is subsequently decarboxylated to benzoyl-CoA by a putative o-phthalyl-CoA decarboxylase. Results from in vitro enzyme assays with cell-free extracts of o-phthalate-grown cells demonstrated the formation of o-phthalyl-CoA from o-phthalate and succinyl-CoA as CoA donor, and its subsequent decarboxylation to benzoyl-CoA. The putative succinyl-CoA:o-phthalate CoA-transferase showed high substrate specificity for o-phthalate and did not accept isophthalate, terephthalate or 3-fluoro-o-phthalate whereas the putative o-phthalyl-CoA decarboxylase converted fluoro-o-phthalyl-CoA to fluoro-benzoyl-CoA. No decarboxylase activity was observed with isophthalyl-CoA or terephthalyl-CoA. Both enzyme activities were oxygen-insensitive and inducible only after growth with o-phthalate. Further degradation of benzoyl-CoA proceeds analogous to the well-established anaerobic benzoyl-CoA degradation pathway of nitrate-reducing bacteria.

Fachgebiet (DDC)

570 Biowissenschaften, Biologie

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ISO 690

JUNGHARE, Madan, Dieter SPITELLER, Bernhard SCHINK, 2016. Enzymes involved in the anaerobic degradation of ortho-phthalate by the nitrate-reducing bacterium Azoarcus sp. strain PA01. In: Environmental Microbiology. 2016, 18(9), pp. 3175-3188. ISSN 1462-2912. eISSN 1462-2920. Available under: doi: 10.1111/1462-2920.13447

BibTex

@article{Junghare2016Enzym-36511,
  year={2016},
  doi={10.1111/1462-2920.13447},
  title={Enzymes involved in the anaerobic degradation of ortho-phthalate by the nitrate-reducing bacterium Azoarcus sp. strain PA01},
  number={9},
  volume={18},
  issn={1462-2912},
  journal={Environmental Microbiology},
  pages={3175--3188},
  author={Junghare, Madan and Spiteller, Dieter and Schink, Bernhard}
}

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