Single-Cell RNA-Sequencing Identifies Activation of TP53 and STAT1 Pathways in Human T Lymphocyte Subpopulations in Response to Ex Vivo Radiation Exposure

Lade...
Vorschaubild
Dateien
Moreno-Villanueva_2-lkfyl8au80p30.pdf
Moreno-Villanueva_2-lkfyl8au80p30.pdfGröße: 1.2 MBDownloads: 341
Datum
2019
Autor:innen
Zhang, Ye
Feiveson, Alan
Mistretta, Brandon
Pan, Yinghong
Chatterjee, Sujash
Wu, Winston
Clanton, Ryan
Nelman-Gonzalez, Mayra
Krieger, Stephanie
Herausgeber:innen
Kontakt
ISSN der Zeitschrift
Electronic ISSN
ISBN
Bibliografische Daten
Verlag
Schriftenreihe
Auflagebezeichnung
ArXiv-ID
Internationale Patentnummer
Link zur Lizenz
Angaben zur Forschungsförderung
Projekt
Open Access-Veröffentlichung
Open Access Gold
Sammlungen
Core Facility der Universität Konstanz
Gesperrt bis
Titel in einer weiteren Sprache
Forschungsvorhaben
Organisationseinheiten
Zeitschriftenheft
Publikationstyp
Zeitschriftenartikel
Publikationsstatus
Published
Erschienen in
International journal of molecular sciences. 2019, 20(9), 2316. eISSN 1422-0067. Available under: doi: 10.3390/ijms20092316
Zusammenfassung

Detrimental health consequences from exposure to space radiation are a major concern for long-duration human exploration missions to the Moon or Mars. Cellular responses to radiation are expected to be heterogeneous for space radiation exposure, where only high-energy protons and other particles traverse a fraction of the cells. Therefore, assessing DNA damage and DNA damage response in individual cells is crucial in understanding the mechanisms by which cells respond to different particle types and energies in space. In this project, we identified a cell-specific signature for radiation response by using single-cell transcriptomics of human lymphocyte subpopulations. We investigated gene expression in individual human T lymphocytes 3 h after ex vivo exposure to 2-Gy gamma rays while using the single-cell sequencing technique (10X Genomics). In the process, RNA was isolated from ~700 irradiated and ~700 non-irradiated control cells, and then sequenced with ~50 k reads/cell. RNA in each of the cells was distinctively barcoded prior to extraction to allow for quantification for individual cells. Principal component and clustering analysis of the unique molecular identifier (UMI) counts classified the cells into three groups or sub-types, which correspond to CD4+, naïve, and CD8+/NK cells. Gene expression changes after radiation exposure were evaluated using negative binomial regression. On average, BBC3, PCNA, and other TP53 related genes that are known to respond to radiation in human T cells showed increased activation. While most of the TP53 responsive genes were upregulated in all groups of cells, the expressions of IRF1, STAT1, and BATF were only upregulated in the CD4+ and naïve groups, but were unchanged in the CD8+/NK group, which suggests that the interferon-gamma pathway does not respond to radiation in CD8+/NK cells. Thus, single-cell RNA sequencing technique was useful for simultaneously identifying the expression of a set of genes in individual cells and T lymphocyte subpopulation after gamma radiation exposure. The degree of dependence of UMI counts between pairs of upregulated genes was also evaluated to construct a similarity matrix for cluster analysis. The cluster analysis identified a group of TP53-responsive genes and a group of genes that are involved in the interferon gamma pathway, which demonstrate the potential of this method for identifying previously unknown groups of genes with similar expression patterns.

Zusammenfassung in einer weiteren Sprache
Fachgebiet (DDC)
570 Biowissenschaften, Biologie
Schlagwörter
single-cell RNA-sequencing; radiation; human T cells
Konferenz
Rezension
undefined / . - undefined, undefined
Zitieren
ISO 690MORENO-VILLANUEVA, Maria, Ye ZHANG, Alan FEIVESON, Brandon MISTRETTA, Yinghong PAN, Sujash CHATTERJEE, Winston WU, Ryan CLANTON, Mayra NELMAN-GONZALEZ, Stephanie KRIEGER, Preethi GUNARATNE, Brian CRUCIAN, Honglu WU, 2019. Single-Cell RNA-Sequencing Identifies Activation of TP53 and STAT1 Pathways in Human T Lymphocyte Subpopulations in Response to Ex Vivo Radiation Exposure. In: International journal of molecular sciences. 2019, 20(9), 2316. eISSN 1422-0067. Available under: doi: 10.3390/ijms20092316
BibTex
@article{MorenoVillanueva2019-05-10Singl-46465,
  year={2019},
  doi={10.3390/ijms20092316},
  title={Single-Cell RNA-Sequencing Identifies Activation of TP53 and STAT1 Pathways in Human T Lymphocyte Subpopulations in Response to Ex Vivo Radiation Exposure},
  number={9},
  volume={20},
  journal={International journal of molecular sciences},
  author={Moreno-Villanueva, Maria and Zhang, Ye and Feiveson, Alan and Mistretta, Brandon and Pan, Yinghong and Chatterjee, Sujash and Wu, Winston and Clanton, Ryan and Nelman-Gonzalez, Mayra and Krieger, Stephanie and Gunaratne, Preethi and Crucian, Brian and Wu, Honglu},
  note={Article Number: 2316}
}
RDF
<rdf:RDF
    xmlns:dcterms="http://purl.org/dc/terms/"
    xmlns:dc="http://purl.org/dc/elements/1.1/"
    xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#"
    xmlns:bibo="http://purl.org/ontology/bibo/"
    xmlns:dspace="http://digital-repositories.org/ontologies/dspace/0.1.0#"
    xmlns:foaf="http://xmlns.com/foaf/0.1/"
    xmlns:void="http://rdfs.org/ns/void#"
    xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > 
  <rdf:Description rdf:about="https://kops.uni-konstanz.de/server/rdf/resource/123456789/46465">
    <dc:creator>Clanton, Ryan</dc:creator>
    <dc:contributor>Moreno-Villanueva, Maria</dc:contributor>
    <dc:language>eng</dc:language>
    <dspace:isPartOfCollection rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/28"/>
    <dc:creator>Krieger, Stephanie</dc:creator>
    <dc:creator>Zhang, Ye</dc:creator>
    <dc:creator>Crucian, Brian</dc:creator>
    <dc:creator>Nelman-Gonzalez, Mayra</dc:creator>
    <dc:contributor>Clanton, Ryan</dc:contributor>
    <dc:contributor>Wu, Winston</dc:contributor>
    <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2019-07-18T14:23:12Z</dc:date>
    <dc:creator>Chatterjee, Sujash</dc:creator>
    <dc:creator>Wu, Winston</dc:creator>
    <dc:creator>Mistretta, Brandon</dc:creator>
    <bibo:uri rdf:resource="https://kops.uni-konstanz.de/handle/123456789/46465"/>
    <dcterms:hasPart rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/46465/1/Moreno-Villanueva_2-lkfyl8au80p30.pdf"/>
    <dcterms:issued>2019-05-10</dcterms:issued>
    <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2019-07-18T14:23:12Z</dcterms:available>
    <dc:contributor>Feiveson, Alan</dc:contributor>
    <dcterms:isPartOf rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/28"/>
    <foaf:homepage rdf:resource="http://localhost:8080/"/>
    <dcterms:rights rdf:resource="http://creativecommons.org/licenses/by/4.0/"/>
    <dspace:hasBitstream rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/46465/1/Moreno-Villanueva_2-lkfyl8au80p30.pdf"/>
    <dc:contributor>Pan, Yinghong</dc:contributor>
    <dc:contributor>Wu, Honglu</dc:contributor>
    <dc:contributor>Nelman-Gonzalez, Mayra</dc:contributor>
    <dc:creator>Moreno-Villanueva, Maria</dc:creator>
    <dc:contributor>Zhang, Ye</dc:contributor>
    <dc:creator>Wu, Honglu</dc:creator>
    <dc:rights>Attribution 4.0 International</dc:rights>
    <dcterms:title>Single-Cell RNA-Sequencing Identifies Activation of TP53 and STAT1 Pathways in Human T Lymphocyte Subpopulations in Response to Ex Vivo Radiation Exposure</dcterms:title>
    <dcterms:abstract xml:lang="eng">Detrimental health consequences from exposure to space radiation are a major concern for long-duration human exploration missions to the Moon or Mars. Cellular responses to radiation are expected to be heterogeneous for space radiation exposure, where only high-energy protons and other particles traverse a fraction of the cells. Therefore, assessing DNA damage and DNA damage response in individual cells is crucial in understanding the mechanisms by which cells respond to different particle types and energies in space. In this project, we identified a cell-specific signature for radiation response by using single-cell transcriptomics of human lymphocyte subpopulations. We investigated gene expression in individual human T lymphocytes 3 h after ex vivo exposure to 2-Gy gamma rays while using the single-cell sequencing technique (10X Genomics). In the process, RNA was isolated from ~700 irradiated and ~700 non-irradiated control cells, and then sequenced with ~50 k reads/cell. RNA in each of the cells was distinctively barcoded prior to extraction to allow for quantification for individual cells. Principal component and clustering analysis of the unique molecular identifier (UMI) counts classified the cells into three groups or sub-types, which correspond to CD4+, naïve, and CD8+/NK cells. Gene expression changes after radiation exposure were evaluated using negative binomial regression. On average, BBC3, PCNA, and other TP53 related genes that are known to respond to radiation in human T cells showed increased activation. While most of the TP53 responsive genes were upregulated in all groups of cells, the expressions of IRF1, STAT1, and BATF were only upregulated in the CD4+ and naïve groups, but were unchanged in the CD8+/NK group, which suggests that the interferon-gamma pathway does not respond to radiation in CD8+/NK cells. Thus, single-cell RNA sequencing technique was useful for simultaneously identifying the expression of a set of genes in individual cells and T lymphocyte subpopulation after gamma radiation exposure. The degree of dependence of UMI counts between pairs of upregulated genes was also evaluated to construct a similarity matrix for cluster analysis. The cluster analysis identified a group of TP53-responsive genes and a group of genes that are involved in the interferon gamma pathway, which demonstrate the potential of this method for identifying previously unknown groups of genes with similar expression patterns.</dcterms:abstract>
    <dc:contributor>Gunaratne, Preethi</dc:contributor>
    <dc:creator>Pan, Yinghong</dc:creator>
    <dc:creator>Gunaratne, Preethi</dc:creator>
    <dc:contributor>Mistretta, Brandon</dc:contributor>
    <void:sparqlEndpoint rdf:resource="http://localhost/fuseki/dspace/sparql"/>
    <dc:contributor>Crucian, Brian</dc:contributor>
    <dc:contributor>Chatterjee, Sujash</dc:contributor>
    <dc:creator>Feiveson, Alan</dc:creator>
    <dc:contributor>Krieger, Stephanie</dc:contributor>
  </rdf:Description>
</rdf:RDF>
Interner Vermerk
xmlui.Submission.submit.DescribeStep.inputForms.label.kops_note_fromSubmitter
Kontakt
URL der Originalveröffentl.
Prüfdatum der URL
Prüfungsdatum der Dissertation
Finanzierungsart
Kommentar zur Publikation
Allianzlizenz
Corresponding Authors der Uni Konstanz vorhanden
Internationale Co-Autor:innen
Universitätsbibliographie
Ja
Begutachtet
Ja
Diese Publikation teilen