A genomic cluster containing novel and conserved genes is associated with cichlid fish dental developmental convergence

Karagic, Nidal; Schneider, Ralf F.; Meyer, Axel; Hulsey, Christopher Darrin

A genomic cluster containing novel and conserved genes is associated with cichlid fish dental developmental convergence

Dateien

Karagic_2-mi75koz3hi2s3.pdfGröße: 2.63 MBDownloads: 113

Datum

2020

Angaben zur Forschungsförderung

European Union (EU): 293700

Open Access-Veröffentlichung

Open Access Green

Sammlungen

Biologie

Publikationstyp

Zeitschriftenartikel

Publikationsstatus

Published

Erschienen in

Molecular Biology and Evolution. Oxford University Press (OUP). 2020, 37(11), pp. 3165-3174. ISSN 0737-4038. eISSN 1537-1719. Available under: doi: 10.1093/molbev/msaa153

Zusammenfassung

The two toothed jaws of cichlid fishes provide textbook examples of convergent evolution. Tooth phenotypes such as enlarged molar-like teeth used to process hard-shelled molluscs have evolved numerous times independently during cichlid diversification. While the ecological benefit of molar-like teeth to crush prey is known, it is unclear whether the same molecular mechanisms underlie these convergent traits. To identify genes involved in the evolution and development of enlarged cichlid teeth, we performed RNA-seq on the serially homologous toothed oral and pharyngeal jaws as well as the fourth toothless gill arch of Astatoreochromis alluaudi. We identified 27 genes that are highly upregulated on both tooth-bearing jaws compared to the toothless gill arch. Most of these genes have never been reported to play a role in tooth formation. Two of these genes (unk, rpfA) are not found in other vertebrate genomes but are present in all cichlid genomes. They also cluster genomically with two other highly expressed tooth genes (odam, scpp5) that exhibit conserved expression during vertebrate odontogenesis. Unk and rpfA were confirmed via in situ hybridization to be expressed in developing teeth of Astatotilapia burtoni. We then examined expression of the cluster's four genes in six evolutionarily independent and phylogenetically disparate cichlid species pairs each with a large- and a small-toothed species. Odam and unk commonly and scpp5 and rpfA always showed higher expression in larger-toothed cichlid jaws. Convergent trophic adaptations across cichlid diversity are associated with the repeated developmental deployment of this genomic cluster containing conserved and novel cichlid-specific genes.

Fachgebiet (DDC)

570 Biowissenschaften, Biologie

Schlagwörter

Cichlidae, pharyngeal jaw, gene birth, odontogenesis, key innovation, evolvability

Zitieren

ISO 690

KARAGIC, Nidal, Ralf F. SCHNEIDER, Axel MEYER, Christopher Darrin HULSEY, 2020. A genomic cluster containing novel and conserved genes is associated with cichlid fish dental developmental convergence. In: Molecular Biology and Evolution. Oxford University Press (OUP). 2020, 37(11), pp. 3165-3174. ISSN 0737-4038. eISSN 1537-1719. Available under: doi: 10.1093/molbev/msaa153

BibTex

@article{Karagic2020-11-01genom-50056,
  year={2020},
  doi={10.1093/molbev/msaa153},
  title={A genomic cluster containing novel and conserved genes is associated with cichlid fish dental developmental convergence},
  number={11},
  volume={37},
  issn={0737-4038},
  journal={Molecular Biology and Evolution},
  pages={3165--3174},
  author={Karagic, Nidal and Schneider, Ralf F. and Meyer, Axel and Hulsey, Christopher Darrin}
}

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