Combining the Sensitivity of LAMP and Simplicity of Primer Extension via a DNA-Modified Nucleotide

Welter, Moritz; Marx, Andreas

Combining the Sensitivity of LAMP and Simplicity of Primer Extension via a DNA-Modified Nucleotide

Dateien

Welter_2-pl43enl37msc9.pdfGröße: 659.19 KBDownloads: 268

Datum

2020

Open Access-Veröffentlichung

Open Access Gold

Sammlungen

Chemie

Publikationstyp

Zeitschriftenartikel

Publikationsstatus

Published

Erschienen in

Chemistry. MDPI. 2020, 2(2), pp. 490-498. eISSN 2624-8549. Available under: doi: 10.3390/chemistry2020029

Zusammenfassung

LAMP is an approach for isothermal nucleic acids diagnostics with increasing importance but suffers from the need of tedious systems design and optimization for every new target. Here, we describe an approach for its simplification based on a single nucleoside-5′-O-triphosphate (dNTP) that is covalently modified with a DNA strand. We found that the DNA-modified dNTP is a substrate for DNA polymerases in versatile primer extension reactions despite its size and that the incorporated DNA indeed serves as a target for selective LAMP analysis.

Fachgebiet (DDC)

540 Chemie

Schlagwörter

modified nucleotide; DNA polymerase; LAMP; primer extension

Zitieren

ISO 690

WELTER, Moritz, Andreas MARX, 2020. Combining the Sensitivity of LAMP and Simplicity of Primer Extension via a DNA-Modified Nucleotide. In: Chemistry. MDPI. 2020, 2(2), pp. 490-498. eISSN 2624-8549. Available under: doi: 10.3390/chemistry2020029

BibTex

@article{Welter2020-06Combi-52032,
  year={2020},
  doi={10.3390/chemistry2020029},
  title={Combining the Sensitivity of LAMP and Simplicity of Primer Extension via a DNA-Modified Nucleotide},
  number={2},
  volume={2},
  journal={Chemistry},
  pages={490--498},
  author={Welter, Moritz and Marx, Andreas}
}

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