Ammonia Released by Streptomyces aburaviensis Induces Droplet Formation in Streptomyces violaceoruber

Schindl, Kathrin; Spiteller, Dieter

Ammonia Released by Streptomyces aburaviensis Induces Droplet Formation in Streptomyces violaceoruber

Dateien

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Datum

2017

Open Access-Veröffentlichung

Open Access Green

Sammlungen

Biologie

Publikationstyp

Zeitschriftenartikel

Publikationsstatus

Published

Erschienen in

Journal of Chemical Ecology. 2017, 43(8), pp. 806-816. ISSN 0098-0331. eISSN 1573-1561. Available under: doi: 10.1007/s10886-017-0870-8

Zusammenfassung

Streptomyces violaceoruber grown in co-culture with Streptomyces aburaviensis produces an about 17-fold higher volume of droplets on its aerial mycelium than in single-culture. Physical separation of the Streptomyces strains by either a plastic barrier or by a dialysis membrane, which allowed communication only by the exchange of volatile compounds or diffusible compounds in the medium, respectively, still resulted in enhanced droplet formation. The application of molecular sieves to bioassays resulted in the attenuation of the droplet-inducing effect of S. aburaviensis indicating the absorption of the compound. 1H-NMR analysis of molecular-sieve extracts and the selective indophenol-blue reaction revealed that the volatile droplet-inducing compound is ammonia. The external supply of ammonia in biologically relevant concentrations of ≥8 mM enhanced droplet formation in S. violaceoruber in a similar way to S. aburaviensis. Ammonia appears to trigger droplet production in many Streptomyces strains because four out of six Streptomyces strains exposed to ammonia exhibited induced droplet production.

Fachgebiet (DDC)

570 Biowissenschaften, Biologie

Schlagwörter

Alkalinisation, Co-cultivation, Microbe-microbe interactions, Morphological differentiation, Volatiles

Zitieren

ISO 690

SCHINDL, Kathrin, Dieter SPITELLER, 2017. Ammonia Released by Streptomyces aburaviensis Induces Droplet Formation in Streptomyces violaceoruber. In: Journal of Chemical Ecology. 2017, 43(8), pp. 806-816. ISSN 0098-0331. eISSN 1573-1561. Available under: doi: 10.1007/s10886-017-0870-8

BibTex

@article{Schindl2017-08Ammon-40057,
  year={2017},
  doi={10.1007/s10886-017-0870-8},
  title={Ammonia Released by Streptomyces aburaviensis Induces Droplet Formation in Streptomyces violaceoruber},
  number={8},
  volume={43},
  issn={0098-0331},
  journal={Journal of Chemical Ecology},
  pages={806--816},
  author={Schindl, Kathrin and Spiteller, Dieter}
}

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