Stabilization of detergent-solubilized Ca2+ -ATPase by poly(ethylene glycol)

Lade...
Vorschaubild
Dateien
Welte_208757.pdf
Welte_208757.pdfGröße: 890.13 KBDownloads: 351
Datum
1989
Autor:innen
Leonhard, Monika
Weltzien, Hans-Ulrich
Restall, Colin
Hall, Christine
Chapman, Dennis
Herausgeber:innen
Kontakt
ISSN der Zeitschrift
Electronic ISSN
ISBN
Bibliografische Daten
Verlag
Schriftenreihe
Auflagebezeichnung
ArXiv-ID
Internationale Patentnummer
Angaben zur Forschungsförderung
Projekt
Open Access-Veröffentlichung
Open Access Green
Sammlungen
Core Facility der Universität Konstanz
Gesperrt bis
Titel in einer weiteren Sprache
Forschungsvorhaben
Organisationseinheiten
Zeitschriftenheft
Publikationstyp
Zeitschriftenartikel
Publikationsstatus
Published
Erschienen in
Biochimica et Biophysica Acta (BBA) - Biomembranes. 1989, 984(2), pp. 193-199. ISSN 0005-2736. Available under: doi: 10.1016/0005-2736(89)90216-2
Zusammenfassung

The (Ca2++Mg2+)-ATPase from sarcoplasmic reticulum (SR) has been solubilized with 1-alkanoyl propanediol 3-phosphorylcholines with chainlengths ranging between 8 and 12 C atoms. A marked dependence of the ATPase activity upon the chainlength was found, indicating that alkyl chainlengths with 12 C atoms are necessary for retention of activity. Addition of poly(ethylene glycol) to the eluting buffers used for gel filtration of the ATPase-detergent micelles was found to increase the activity and the long-term stability significantly. In the presence of Ca2+, the elution volume indicated an ATPase dimer, whereas in the absence of Ca2+ the elution volume indicated a monomeric solution. The purity of the preparations after gel filtration was improved by subsequent chromatography with a hydroxyapatite column.

Zusammenfassung in einer weiteren Sprache
Fachgebiet (DDC)
570 Biowissenschaften, Biologie
Schlagwörter
ATPase, Ca2+-, enzyme stability, detergent, poly(ethylene glycol), (sarcoplasmic reticulum)
Konferenz
Rezension
undefined / . - undefined, undefined
Zitieren
ISO 690WELTE, Wolfram, Monika LEONHARD, Kay DIEDERICHS, Hans-Ulrich WELTZIEN, Colin RESTALL, Christine HALL, Dennis CHAPMAN, 1989. Stabilization of detergent-solubilized Ca2+ -ATPase by poly(ethylene glycol). In: Biochimica et Biophysica Acta (BBA) - Biomembranes. 1989, 984(2), pp. 193-199. ISSN 0005-2736. Available under: doi: 10.1016/0005-2736(89)90216-2
BibTex
@article{Welte1989Stabi-20875,
  year={1989},
  doi={10.1016/0005-2736(89)90216-2},
  title={Stabilization of detergent-solubilized Ca2+ -ATPase by poly(ethylene glycol)},
  number={2},
  volume={984},
  issn={0005-2736},
  journal={Biochimica et Biophysica Acta (BBA) - Biomembranes},
  pages={193--199},
  author={Welte, Wolfram and Leonhard, Monika and Diederichs, Kay and Weltzien, Hans-Ulrich and Restall, Colin and Hall, Christine and Chapman, Dennis}
}
RDF
<rdf:RDF
    xmlns:dcterms="http://purl.org/dc/terms/"
    xmlns:dc="http://purl.org/dc/elements/1.1/"
    xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#"
    xmlns:bibo="http://purl.org/ontology/bibo/"
    xmlns:dspace="http://digital-repositories.org/ontologies/dspace/0.1.0#"
    xmlns:foaf="http://xmlns.com/foaf/0.1/"
    xmlns:void="http://rdfs.org/ns/void#"
    xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > 
  <rdf:Description rdf:about="https://kops.uni-konstanz.de/server/rdf/resource/123456789/20875">
    <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2012-11-19T07:18:39Z</dcterms:available>
    <dc:contributor>Chapman, Dennis</dc:contributor>
    <dc:creator>Restall, Colin</dc:creator>
    <dc:contributor>Restall, Colin</dc:contributor>
    <dc:language>eng</dc:language>
    <dc:contributor>Diederichs, Kay</dc:contributor>
    <dspace:isPartOfCollection rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/28"/>
    <dcterms:bibliographicCitation>Biochimica et Biophysica Acta (BBA) - Biomembranes ; 984 (1989), 2. - S. 193-199</dcterms:bibliographicCitation>
    <dc:creator>Leonhard, Monika</dc:creator>
    <dc:creator>Weltzien, Hans-Ulrich</dc:creator>
    <dc:creator>Hall, Christine</dc:creator>
    <dc:rights>terms-of-use</dc:rights>
    <dc:contributor>Hall, Christine</dc:contributor>
    <dspace:hasBitstream rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/20875/2/Welte_208757.pdf"/>
    <dcterms:isPartOf rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/28"/>
    <dcterms:hasPart rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/20875/2/Welte_208757.pdf"/>
    <dc:contributor>Leonhard, Monika</dc:contributor>
    <foaf:homepage rdf:resource="http://localhost:8080/"/>
    <dc:contributor>Weltzien, Hans-Ulrich</dc:contributor>
    <bibo:uri rdf:resource="http://kops.uni-konstanz.de/handle/123456789/20875"/>
    <dc:creator>Chapman, Dennis</dc:creator>
    <void:sparqlEndpoint rdf:resource="http://localhost/fuseki/dspace/sparql"/>
    <dc:creator>Welte, Wolfram</dc:creator>
    <dc:creator>Diederichs, Kay</dc:creator>
    <dcterms:title>Stabilization of detergent-solubilized Ca2+ -ATPase by poly(ethylene glycol)</dcterms:title>
    <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2012-11-19T07:18:39Z</dc:date>
    <dc:contributor>Welte, Wolfram</dc:contributor>
    <dcterms:abstract xml:lang="eng">The (Ca2++Mg2+)-ATPase from sarcoplasmic reticulum (SR) has been solubilized with 1-alkanoyl propanediol 3-phosphorylcholines with chainlengths ranging between 8 and 12 C atoms. A marked dependence of the ATPase activity upon the chainlength was found, indicating that alkyl chainlengths with 12 C atoms are necessary for retention of activity. Addition of poly(ethylene glycol) to the eluting buffers used for gel filtration of the ATPase-detergent micelles was found to increase the activity and the long-term stability significantly. In the presence of Ca2+, the elution volume indicated an ATPase dimer, whereas in the absence of Ca2+ the elution volume indicated a monomeric solution. The purity of the preparations after gel filtration was improved by subsequent chromatography with a hydroxyapatite column.</dcterms:abstract>
    <dcterms:issued>1989</dcterms:issued>
    <dcterms:rights rdf:resource="https://rightsstatements.org/page/InC/1.0/"/>
  </rdf:Description>
</rdf:RDF>
Interner Vermerk
xmlui.Submission.submit.DescribeStep.inputForms.label.kops_note_fromSubmitter
Kontakt
URL der Originalveröffentl.
Prüfdatum der URL
Prüfungsdatum der Dissertation
Finanzierungsart
Kommentar zur Publikation
Allianzlizenz
Corresponding Authors der Uni Konstanz vorhanden
Internationale Co-Autor:innen
Universitätsbibliographie
Nein
Begutachtet
Diese Publikation teilen