Giant lipid vesicles under electric field pulses assessed by non invasive imaging

Lade...
Vorschaubild
Dateien
Mauroy_211222.pdf
Mauroy_211222.pdfGrĆ¶ĆŸe: 1.37 MBDownloads: 451
Datum
2012
Autor:innen
Mauroy, ChloƩ
Portet, Thomas
Bellard, Elisabeth
Blache, Marie-Claire
TeissiƩ, Justin
Rols, Marie-Pierre
Herausgeber:innen
Kontakt
ISSN der Zeitschrift
Electronic ISSN
ISBN
Bibliografische Daten
Verlag
Schriftenreihe
Auflagebezeichnung
ArXiv-ID
Internationale Patentnummer
Angaben zur Forschungsfƶrderung
Projekt
Open Access-Verƶffentlichung
Open Access Green
Sammlungen
Core Facility der UniversitƤt Konstanz
Gesperrt bis
Titel in einer weiteren Sprache
Forschungsvorhaben
Organisationseinheiten
Zeitschriftenheft
Publikationstyp
Zeitschriftenartikel
Publikationsstatus
Published
Erschienen in
Bioelectrochemistry. 2012, 87, pp. 253-259. ISSN 1567-5394. eISSN 1878-562X. Available under: doi: 10.1016/j.bioelechem.2012.03.008
Zusammenfassung

We present experimental results regarding the effects of electric pulses on giant unilamellar vesicles (GUVs). We have used phase contrast and coherent anti-Stokes Raman scattering (CARS) microscopy as relevant optical approaches to gain insight into membrane changes under electropermeabilization. No addition of exogenous molecules (lipid analogue, fluorescent dye) was needed. Therefore, experiments were performed on pure lipid systems avoiding possible artefacts linked to their use. Structural membrane changes were assessed by loss of contrast inside the GUVs due to sucrose and glucose mixing. Our observations, performed at the single vesicle level, indicate these changes are under the control of the number of pulses and field intensity. Larger number of pulses enhances membrane alterations. A threshold value of the field intensity must be applied to allow exchange of molecules between GUVs and the external medium. This threshold depends on the size of the vesicles, the larger GUVs being affected at lower electric field strengths than the smaller ones. Our experimental data are well described by a simple model in which molecule entry is driven by direct exchange. The CARS microscopic study of the effect of pulse duration confirms that pulses, in the ms time range, induce loss of lipids and membrane deformations facing the electrodes.

Zusammenfassung in einer weiteren Sprache
Fachgebiet (DDC)
540 Chemie
Schlagwƶrter
Pulsed electric fields, Electroporation, Imaging, Phospholipids
Konferenz
Rezension
undefined / . - undefined, undefined
Zitieren
ISO 690MAUROY, ChloĆ©, Thomas PORTET, Martin WINTERHALDER, Elisabeth BELLARD, Marie-Claire BLACHE, Justin TEISSIƉ, Andreas ZUMBUSCH, Marie-Pierre ROLS, 2012. Giant lipid vesicles under electric field pulses assessed by non invasive imaging. In: Bioelectrochemistry. 2012, 87, pp. 253-259. ISSN 1567-5394. eISSN 1878-562X. Available under: doi: 10.1016/j.bioelechem.2012.03.008
BibTex
@article{Mauroy2012-10Giant-21122,
  year={2012},
  doi={10.1016/j.bioelechem.2012.03.008},
  title={Giant lipid vesicles under electric field pulses assessed by non invasive imaging},
  volume={87},
  issn={1567-5394},
  journal={Bioelectrochemistry},
  pages={253--259},
  author={Mauroy, ChloƩ and Portet, Thomas and Winterhalder, Martin and Bellard, Elisabeth and Blache, Marie-Claire and TeissiƩ, Justin and Zumbusch, Andreas and Rols, Marie-Pierre}
}
RDF
<rdf:RDF
    xmlns:dcterms="http://purl.org/dc/terms/"
    xmlns:dc="http://purl.org/dc/elements/1.1/"
    xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#"
    xmlns:bibo="http://purl.org/ontology/bibo/"
    xmlns:dspace="http://digital-repositories.org/ontologies/dspace/0.1.0#"
    xmlns:foaf="http://xmlns.com/foaf/0.1/"
    xmlns:void="http://rdfs.org/ns/void#"
    xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > 
  <rdf:Description rdf:about="https://kops.uni-konstanz.de/server/rdf/resource/123456789/21122">
    <dc:contributor>Portet, Thomas</dc:contributor>
    <dc:creator>Blache, Marie-Claire</dc:creator>
    <dspace:isPartOfCollection rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/29"/>
    <bibo:uri rdf:resource="http://kops.uni-konstanz.de/handle/123456789/21122"/>
    <dc:contributor>Mauroy, ChloƩ</dc:contributor>
    <dcterms:bibliographicCitation>Bioelectrochemistry ; 87 (2012). - S. 253-259</dcterms:bibliographicCitation>
    <dc:rights>terms-of-use</dc:rights>
    <dc:contributor>Bellard, Elisabeth</dc:contributor>
    <dc:creator>Winterhalder, Martin</dc:creator>
    <dc:creator>Zumbusch, Andreas</dc:creator>
    <dspace:hasBitstream rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/21122/2/Mauroy_211222.pdf"/>
    <dcterms:isPartOf rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/29"/>
    <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2012-12-19T09:32:46Z</dcterms:available>
    <dc:contributor>Zumbusch, Andreas</dc:contributor>
    <foaf:homepage rdf:resource="http://localhost:8080/"/>
    <dcterms:hasPart rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/21122/2/Mauroy_211222.pdf"/>
    <dc:creator>TeissiƩ, Justin</dc:creator>
    <dc:contributor>TeissiƩ, Justin</dc:contributor>
    <dc:creator>Rols, Marie-Pierre</dc:creator>
    <dcterms:issued>2012-10</dcterms:issued>
    <dc:creator>Bellard, Elisabeth</dc:creator>
    <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2012-12-19T09:32:46Z</dc:date>
    <dc:language>eng</dc:language>
    <dcterms:title>Giant lipid vesicles under electric field pulses assessed by non invasive imaging</dcterms:title>
    <dc:contributor>Rols, Marie-Pierre</dc:contributor>
    <dc:contributor>Winterhalder, Martin</dc:contributor>
    <dc:creator>Mauroy, ChloƩ</dc:creator>
    <dc:contributor>Blache, Marie-Claire</dc:contributor>
    <void:sparqlEndpoint rdf:resource="http://localhost/fuseki/dspace/sparql"/>
    <dc:creator>Portet, Thomas</dc:creator>
    <dcterms:abstract xml:lang="eng">We present experimental results regarding the effects of electric pulses on giant unilamellar vesicles (GUVs). We have used phase contrast and coherent anti-Stokes Raman scattering (CARS) microscopy as relevant optical approaches to gain insight into membrane changes under electropermeabilization. No addition of exogenous molecules (lipid analogue, fluorescent dye) was needed. Therefore, experiments were performed on pure lipid systems avoiding possible artefacts linked to their use. Structural membrane changes were assessed by loss of contrast inside the GUVs due to sucrose and glucose mixing. Our observations, performed at the single vesicle level, indicate these changes are under the control of the number of pulses and field intensity. Larger number of pulses enhances membrane alterations. A threshold value of the field intensity must be applied to allow exchange of molecules between GUVs and the external medium. This threshold depends on the size of the vesicles, the larger GUVs being affected at lower electric field strengths than the smaller ones. Our experimental data are well described by a simple model in which molecule entry is driven by direct exchange. The CARS microscopic study of the effect of pulse duration confirms that pulses, in the ms time range, induce loss of lipids and membrane deformations facing the electrodes.</dcterms:abstract>
    <dcterms:rights rdf:resource="https://rightsstatements.org/page/InC/1.0/"/>
  </rdf:Description>
</rdf:RDF>
Interner Vermerk
xmlui.Submission.submit.DescribeStep.inputForms.label.kops_note_fromSubmitter
Kontakt
URL der Originalverƶffentl.
PrĆ¼fdatum der URL
PrĆ¼fungsdatum der Dissertation
Finanzierungsart
Kommentar zur Publikation
Allianzlizenz
Corresponding Authors der Uni Konstanz vorhanden
Internationale Co-Autor:innen
UniversitƤtsbibliographie
Ja
Begutachtet
Diese Publikation teilen