Reporter assays for studying quadruplex nucleic acids

Halder, Kangkan; Benzler, Martin; Hartig, Jörg S.

Reporter assays for studying quadruplex nucleic acids

Dateien

halder_211662.pdfGröße: 737.51 KBDownloads: 862

Datum

2012

Autor:innen

Halder, Kangkan

Benzler, Martin

Hartig, Jörg S.

Open Access-Veröffentlichung

Open Access Green

Sammlungen

Chemie

Publikationstyp

Zeitschriftenartikel

Publikationsstatus

Published

Erschienen in

Methods. 2012, 57(1), pp. 115-121. ISSN 1046-2023. eISSN 1095-9130. Available under: doi: 10.1016/j.ymeth.2012.02.005

Zusammenfassung

DNA and RNA G-quadruplexes have gained increasing attention due to their potential role in a wide range of biological functions. The majority of functional studies characterize the influence of quadruplexes in gene expression including transcription and translation. Many of these studies have used reporter assays to elucidate the effect of quadruplexes at certain positions in promoters and untranslated mRNA regions (UTRs). Reporter assays are the preferred method to ascertain the biological function of DNA or RNA G-quadruplexes intracellularly due to their ready availability, fast cloning and experimental setup and reproducibility. Moreover, these reporter assays are also helpful to compare or screen for selectivity and efficacy of small molecules that target DNA and RNA G-quadruplexes in the cellular context. Here we briefly discuss various aspects of reporter assays followed by a review of available studies using reporter assays to understand the role and functions of DNA and RNA quadruplexes in gene expression.

Fachgebiet (DDC)

540 Chemie

Zitieren

ISO 690

HALDER, Kangkan, Martin BENZLER, Jörg S. HARTIG, 2012. Reporter assays for studying quadruplex nucleic acids. In: Methods. 2012, 57(1), pp. 115-121. ISSN 1046-2023. eISSN 1095-9130. Available under: doi: 10.1016/j.ymeth.2012.02.005

BibTex

@article{Halder2012-05Repor-21166,
  year={2012},
  doi={10.1016/j.ymeth.2012.02.005},
  title={Reporter assays for studying quadruplex nucleic acids},
  number={1},
  volume={57},
  issn={1046-2023},
  journal={Methods},
  pages={115--121},
  author={Halder, Kangkan and Benzler, Martin and Hartig, Jörg S.}
}

RDF

<rdf:RDF
    xmlns:dcterms="http://purl.org/dc/terms/"
    xmlns:dc="http://purl.org/dc/elements/1.1/"
    xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#"
    xmlns:bibo="http://purl.org/ontology/bibo/"
    xmlns:dspace="http://digital-repositories.org/ontologies/dspace/0.1.0#"
    xmlns:foaf="http://xmlns.com/foaf/0.1/"
    xmlns:void="http://rdfs.org/ns/void#"
    xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > 
  <rdf:Description rdf:about="https://kops.uni-konstanz.de/server/rdf/resource/123456789/21166">
    <dc:contributor>Benzler, Martin</dc:contributor>
    <dc:rights>terms-of-use</dc:rights>
    <dcterms:hasPart rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/21166/2/halder_211662.pdf"/>
    <dc:contributor>Halder, Kangkan</dc:contributor>
    <dspace:hasBitstream rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/21166/2/halder_211662.pdf"/>
    <dcterms:isPartOf rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/29"/>
    <void:sparqlEndpoint rdf:resource="http://localhost/fuseki/dspace/sparql"/>
    <dc:creator>Benzler, Martin</dc:creator>
    <dcterms:rights rdf:resource="https://rightsstatements.org/page/InC/1.0/"/>
    <bibo:uri rdf:resource="http://kops.uni-konstanz.de/handle/123456789/21166"/>
    <dc:language>eng</dc:language>
    <dcterms:title>Reporter assays for studying quadruplex nucleic acids</dcterms:title>
    <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2013-01-16T08:17:03Z</dc:date>
    <dcterms:abstract>DNA and RNA G-quadruplexes have gained increasing attention due to their potential role in a wide range of biological functions. The majority of functional studies characterize the influence of quadruplexes in gene expression including transcription and translation. Many of these studies have used reporter assays to elucidate the effect of quadruplexes at certain positions in promoters and untranslated mRNA regions (UTRs). Reporter assays are the preferred method to ascertain the biological function of DNA or RNA G-quadruplexes intracellularly due to their ready availability, fast cloning and experimental setup and reproducibility. Moreover, these reporter assays are also helpful to compare or screen for selectivity and efficacy of small molecules that target DNA and RNA G-quadruplexes in the cellular context. Here we briefly discuss various aspects of reporter assays followed by a review of available studies using reporter assays to understand the role and functions of DNA and RNA quadruplexes in gene expression.</dcterms:abstract>
    <dspace:isPartOfCollection rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/29"/>
    <dc:contributor>Hartig, Jörg S.</dc:contributor>
    <dc:creator>Halder, Kangkan</dc:creator>
    <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2013-01-16T08:17:03Z</dcterms:available>
    <dc:creator>Hartig, Jörg S.</dc:creator>
    <dcterms:issued>2012-05</dcterms:issued>
    <foaf:homepage rdf:resource="http://localhost:8080/"/>
    <dcterms:bibliographicCitation>Methods ; 57 (2012), 1. - S. 115-121</dcterms:bibliographicCitation>
  </rdf:Description>
</rdf:RDF>

Universitätsbibliographie

Ja