High yield expression of catalytically active USP18 (UBP43) using a Trigger Factor fusion system

Basters, Anja; Ketscher, Lars; Deuerling, Elke; Arkona, Christoph; Rademann, Jörg; Knobeloch, Klaus-Peter; Fritz, Günter

High yield expression of catalytically active USP18 (UBP43) using a Trigger Factor fusion system

Dateien

basters_214084.pdfGröße: 928.71 KBDownloads: 290

Datum

2012

Autor:innen

Basters, Anja

Ketscher, Lars

Deuerling, Elke

Arkona, Christoph

Rademann, Jörg

Knobeloch, Klaus-Peter

Fritz, Günter

Open Access-Veröffentlichung

Open Access Gold

Sammlungen

Biologie

Publikationstyp

Zeitschriftenartikel

Publikationsstatus

Published

Erschienen in

BMC Biotechnology. 2012, 12(1), 56. eISSN 1472-6750. Available under: doi: 10.1186/1472-6750-12-56

Zusammenfassung

Covalent linkage of the ubiquitin-like protein ISG15 interferes with viral infection and USP18 is the major protease which specifically removes ISG15 from target proteins. Thus, boosting ISG15 modification by protease inhibition of USP18 might represent a new strategy to interfere with viral replication. However, so far no heterologous expression system was available to yield sufficient amounts of catalytically active protein for high-throughput based inhibitor screens.

Fachgebiet (DDC)

570 Biowissenschaften, Biologie

Zitieren

ISO 690

BASTERS, Anja, Lars KETSCHER, Elke DEUERLING, Christoph ARKONA, Jörg RADEMANN, Klaus-Peter KNOBELOCH, Günter FRITZ, 2012. High yield expression of catalytically active USP18 (UBP43) using a Trigger Factor fusion system. In: BMC Biotechnology. 2012, 12(1), 56. eISSN 1472-6750. Available under: doi: 10.1186/1472-6750-12-56

BibTex

@article{Basters2012yield-21408,
  year={2012},
  doi={10.1186/1472-6750-12-56},
  title={High yield expression of catalytically active USP18 (UBP43) using a Trigger Factor fusion system},
  number={1},
  volume={12},
  journal={BMC Biotechnology},
  author={Basters, Anja and Ketscher, Lars and Deuerling, Elke and Arkona, Christoph and Rademann, Jörg and Knobeloch, Klaus-Peter and Fritz, Günter},
  note={Article Number: 56}
}

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Universitätsbibliographie

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