Optical imaging of concealed brain activity using a gold mirror in honeybees

Galizia, C. Giovanni; Franke, Tilman; Menzel, Randolf; Sandoz, Jean-Christophe

Optical imaging of concealed brain activity using a gold mirror in honeybees

Dateien

Galizia_216777.pdfGröße: 1.19 MBDownloads: 200

Datum

2012

Autor:innen

Galizia, C. Giovanni

Franke, Tilman

Menzel, Randolf

Sandoz, Jean-Christophe

Open Access-Veröffentlichung

Open Access Green

Sammlungen

Biologie

Publikationstyp

Zeitschriftenartikel

Publikationsstatus

Published

Erschienen in

Journal of Insect Physiology. 2012, 58(5), pp. 743-749. ISSN 0022-1910. eISSN 1879-1611. Available under: doi: 10.1016/j.jinsphys.2012.02.010

Zusammenfassung

Brain activity is inherently combinatorial and three-dimensional. Optical imaging techniques offer a suitable opportunity to record many activity foci simultaneously, but under conventional microscopy conditions, optical access is generally limited to the frontal part of the brain. Thus, even for cases in which optical recordings have delivered substantial data, our knowledge of deeper layers is deficient. Using the honeybee olfactory system as a test system, we report that by using a gold-sputtered cover slip as a minute mirror, it is possible to optically access and record from otherwise inaccessible brain areas. In insects, the first brain area to code for odors is the antennal lobe (comparable to the vertebrate olfactory bulb). Several previous studies have characterized glomerular odor response patterns of the frontal view, readily accessible when the head capsule of the bee is opened. However, until now, the back and the sides of the antennal lobe have remained utterly unexplored. This is particularly relevant because in the honeybee these two views coincide with two separate olfactory subsystems, related to two axonal tracts of second-order neurons: the lAPT and the mAPT. Combining wide-field microscopy, calcium imaging, and a minute mirror, we report the first glomerular odor responses from the side of the honeybee antennal lobe.

Fachgebiet (DDC)

570 Biowissenschaften, Biologie

Schlagwörter

olfactory coding, calcium imaging, Honeybees

Zitieren

ISO 690

GALIZIA, C. Giovanni, Tilman FRANKE, Randolf MENZEL, Jean-Christophe SANDOZ, 2012. Optical imaging of concealed brain activity using a gold mirror in honeybees. In: Journal of Insect Physiology. 2012, 58(5), pp. 743-749. ISSN 0022-1910. eISSN 1879-1611. Available under: doi: 10.1016/j.jinsphys.2012.02.010

BibTex

@article{Galizia2012-05Optic-21677,
  year={2012},
  doi={10.1016/j.jinsphys.2012.02.010},
  title={Optical imaging of concealed brain activity using a gold mirror in honeybees},
  number={5},
  volume={58},
  issn={0022-1910},
  journal={Journal of Insect Physiology},
  pages={743--749},
  author={Galizia, C. Giovanni and Franke, Tilman and Menzel, Randolf and Sandoz, Jean-Christophe}
}

RDF

<rdf:RDF
    xmlns:dcterms="http://purl.org/dc/terms/"
    xmlns:dc="http://purl.org/dc/elements/1.1/"
    xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#"
    xmlns:bibo="http://purl.org/ontology/bibo/"
    xmlns:dspace="http://digital-repositories.org/ontologies/dspace/0.1.0#"
    xmlns:foaf="http://xmlns.com/foaf/0.1/"
    xmlns:void="http://rdfs.org/ns/void#"
    xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > 
  <rdf:Description rdf:about="https://kops.uni-konstanz.de/server/rdf/resource/123456789/21677">
    <dspace:isPartOfCollection rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/28"/>
    <bibo:uri rdf:resource="http://kops.uni-konstanz.de/handle/123456789/21677"/>
    <dcterms:title>Optical imaging of concealed brain activity using a gold mirror in honeybees</dcterms:title>
    <dcterms:isPartOf rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/28"/>
    <void:sparqlEndpoint rdf:resource="http://localhost/fuseki/dspace/sparql"/>
    <dc:contributor>Sandoz, Jean-Christophe</dc:contributor>
    <dc:contributor>Franke, Tilman</dc:contributor>
    <dc:creator>Sandoz, Jean-Christophe</dc:creator>
    <dspace:hasBitstream rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/21677/2/Galizia_216777.pdf"/>
    <dcterms:issued>2012-05</dcterms:issued>
    <dc:creator>Franke, Tilman</dc:creator>
    <dc:language>eng</dc:language>
    <foaf:homepage rdf:resource="http://localhost:8080/"/>
    <dc:creator>Galizia, C. Giovanni</dc:creator>
    <dc:contributor>Menzel, Randolf</dc:contributor>
    <dc:contributor>Galizia, C. Giovanni</dc:contributor>
    <dc:rights>terms-of-use</dc:rights>
    <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2013-02-14T11:06:10Z</dc:date>
    <dcterms:bibliographicCitation>Journal of Insect Physiology ; 58 (2012), 5. - S. 743-749</dcterms:bibliographicCitation>
    <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2013-02-14T11:06:10Z</dcterms:available>
    <dc:creator>Menzel, Randolf</dc:creator>
    <dcterms:rights rdf:resource="https://rightsstatements.org/page/InC/1.0/"/>
    <dcterms:hasPart rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/21677/2/Galizia_216777.pdf"/>
    <dcterms:abstract xml:lang="eng">Brain activity is inherently combinatorial and three-dimensional. Optical imaging techniques offer a suitable opportunity to record many activity foci simultaneously, but under conventional microscopy conditions, optical access is generally limited to the frontal part of the brain. Thus, even for cases in which optical recordings have delivered substantial data, our knowledge of deeper layers is deficient. Using the honeybee olfactory system as a test system, we report that by using a gold-sputtered cover slip as a minute mirror, it is possible to optically access and record from otherwise inaccessible brain areas. In insects, the first brain area to code for odors is the antennal lobe (comparable to the vertebrate olfactory bulb). Several previous studies have characterized glomerular odor response patterns of the frontal view, readily accessible when the head capsule of the bee is opened. However, until now, the back and the sides of the antennal lobe have remained utterly unexplored. This is particularly relevant because in the honeybee these two views coincide with two separate olfactory subsystems, related to two axonal tracts of second-order neurons: the lAPT and the mAPT. Combining wide-field microscopy, calcium imaging, and a minute mirror, we report the first glomerular odor responses from the side of the honeybee antennal lobe.</dcterms:abstract>
  </rdf:Description>
</rdf:RDF>

Universitätsbibliographie

Ja