Redox regulation of carbonic anhydrases via thioredoxin in the chloroplast of the marine diatom Phaeodactylum tricornutum

Kikutani, Sae; Tanaka, Rie; Yamazaki, Yukiko; Hara, Satoshi; Hisabori, Toru; Kroth, Peter G.; Matsuda, Yusuke

Redox regulation of carbonic anhydrases via thioredoxin in the chloroplast of the marine diatom Phaeodactylum tricornutum

Dateien

Zu diesem Dokument gibt es keine Dateien.

Datum

2012

Autor:innen

Kikutani, Sae

Tanaka, Rie

Yamazaki, Yukiko

Hara, Satoshi

Hisabori, Toru

Kroth, Peter G.

Matsuda, Yusuke

URI (zitierfähiger Link)

http://nbn-resolving.de/urn:nbn:de:bsz:352-219707

DOI (zitierfähiger Link)

https://doi.org/10.1074/jbc.M111.322743

Projekt

TP 5 Light regulation of carbon fixation in diatoms

Sammlungen

Biologie

Publikationstyp

Zeitschriftenartikel

Publikationsstatus

Published

Erschienen in

Journal of Biological Chemistry. 2012, 287(24), pp. 20689-20700. ISSN 0021-9258. eISSN 1083-351X. Available under: doi: 10.1074/jbc.M111.322743

Zusammenfassung

Thioredoxins (Trxs) are important regulators of photosynthetic fixation of CO(2) and nitrogen in plant chloroplasts. To date, they have been considered to play a minor role in controlling the Calvin cycle in marine diatoms, aquatic primary producers, although diatoms possess a set of plastidic Trxs. In this study we examined the influences of the redox state and the involvement of Trxs in the enzymatic activities of pyrenoidal carbonic anhydrases, PtCA1 and PtCA2, in the marine diatom Phaeodactylum tricornutum. The recombinant mature PtCA1 and -2 (mPtCA1 and -2) were completely inactivated following oxidation by 50 μm CuCl(2), whereas DTT activated CAs in a concentration-dependent manner. The maximum activity of mPtCAs in the presence of 6 mm reduced DTT increased significantly by addition of 10 μm Trxs from Arabidopsis thaliana (AtTrx-f2 and -m2) and 5 μm Trxs from P. tricornutum (PtTrxF and -M). Analyses of mPtCA activation by Trxs in the presence of DTT revealed that the maximum mPtCA1 activity was enhanced ∼3-fold in the presence of Trx, whereas mPtCA2 was only weakly activated by Trxs, and that PtTrxs activate PtCAs more efficiently compared with AtTrxs. Site-directed mutagenesis of potential disulfide-forming cysteines in mPtCA1 and mPtCA2 resulted in a lack of oxidative inactivation of both mPtCAs. These results reveal the first direct evidence of a target of plastidic Trxs in diatoms, indicating that Trxs may participate in the redox control of inorganic carbon flow in the pyrenoid, a focal point of the CO(2)-concentrating mechanism.

Fachgebiet (DDC)

570 Biowissenschaften, Biologie

Zitieren

ISO 690

KIKUTANI, Sae, Rie TANAKA, Yukiko YAMAZAKI, Satoshi HARA, Toru HISABORI, Peter G. KROTH, Yusuke MATSUDA, 2012. Redox regulation of carbonic anhydrases via thioredoxin in the chloroplast of the marine diatom Phaeodactylum tricornutum. In: Journal of Biological Chemistry. 2012, 287(24), pp. 20689-20700. ISSN 0021-9258. eISSN 1083-351X. Available under: doi: 10.1074/jbc.M111.322743

BibTex

@article{Kikutani2012-06-08Redox-21970,
  year={2012},
  doi={10.1074/jbc.M111.322743},
  title={Redox regulation of carbonic anhydrases via thioredoxin in the chloroplast of the marine diatom Phaeodactylum tricornutum},
  number={24},
  volume={287},
  issn={0021-9258},
  journal={Journal of Biological Chemistry},
  pages={20689--20700},
  author={Kikutani, Sae and Tanaka, Rie and Yamazaki, Yukiko and Hara, Satoshi and Hisabori, Toru and Kroth, Peter G. and Matsuda, Yusuke}
}

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