Prostaglandin E2 inhibits IL-23 and IL-12 production by human monocytes through down-regulation of their common p40 subunit

Kalim, Khalid W.; Gröttrup, Marcus

Prostaglandin E2 inhibits IL-23 and IL-12 production by human monocytes through down-regulation of their common p40 subunit

Dateien

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Datum

2013

Open Access-Veröffentlichung

Open Access Green

Sammlungen

Biologie

Publikationstyp

Zeitschriftenartikel

Publikationsstatus

Published

Erschienen in

Molecular Immunology. 2013, 53(3), pp. 274-282. ISSN 0161-5890. eISSN 1872-9142. Available under: doi: 10.1016/j.molimm.2012.08.014

Zusammenfassung

The heterodimeric cytokine IL-23 is important for the maintenance of Th17 cells, which are pivotal mediators of autoimmune diseases like rheumatoid arthritis, colitis, and multiple sclerosis. Prostaglandin E2 (PGE2) is a soluble regulator of inflammation that has both pro- and anti-inflammatory properties. PGE2 has been shown to elevate the IL-23 production by dendritic cells (DC). Monocytes are also producers of IL-23 but the effect of PGE2 on IL-23 production by human monocytes has hardly been investigated. We show here that PGE2 blocks the production of IL-23 by LPS-stimulated monocytes in an IL-10 and IL-1 independent manner. This effect was due to the down-regulation of the p40 subunit of IL-23 on mRNA and protein level. The p40 subunit is shared by IL-12 and, consistently, PGE2 also lowered the IL-12 production by monocytes. These effects of PGE2 were cAMP-dependent since the cAMP enhancer forskolin strongly reduced IL-23 and IL-12 production by monocytes. Taken together, PGE2 acts in an anti-inflammatory manner by lowering IL-23 production by monocytes while it has the opposite effect in DC. Our data may help to reconcile controversial point of views on the pro- and anti-inflammatory nature of PGE2 by making a strong case for a cell type-dependent function.

Fachgebiet (DDC)

570 Biowissenschaften, Biologie

Schlagwörter

PGE2, Cytokines, Th17 differentiation, cAMP, ELISA

Zitieren

ISO 690

KALIM, Khalid W., Marcus GRÖTTRUP, 2013. Prostaglandin E2 inhibits IL-23 and IL-12 production by human monocytes through down-regulation of their common p40 subunit. In: Molecular Immunology. 2013, 53(3), pp. 274-282. ISSN 0161-5890. eISSN 1872-9142. Available under: doi: 10.1016/j.molimm.2012.08.014

BibTex

@article{Kalim2013-03Prost-21990,
  year={2013},
  doi={10.1016/j.molimm.2012.08.014},
  title={Prostaglandin E2 inhibits IL-23 and IL-12 production by human monocytes through down-regulation of their common p40 subunit},
  number={3},
  volume={53},
  issn={0161-5890},
  journal={Molecular Immunology},
  pages={274--282},
  author={Kalim, Khalid W. and Gröttrup, Marcus}
}

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