Oxytocin modulates proliferation and stress responses of human skin cells : implications for atopic dermatitis

Deing, Verena; Roggenkamp, Dennis; Kühnl, Jochen; Gruschka, Alisa; Stäb, Franz; Wenck, Horst; Bürkle, Alexander; Neufang, Gitta

Oxytocin modulates proliferation and stress responses of human skin cells : implications for atopic dermatitis

Dateien

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Datum

2013

Autor:innen

Deing, Verena

Roggenkamp, Dennis

Kühnl, Jochen

Gruschka, Alisa

Stäb, Franz

Wenck, Horst

Bürkle, Alexander

Neufang, Gitta

Open Access-Veröffentlichung

Open Access Green

Sammlungen

Biologie

Publikationstyp

Zeitschriftenartikel

Publikationsstatus

Published

Erschienen in

Experimental Dermatology. 2013, 22(6), pp. 399-405. ISSN 0906-6705. eISSN 1600-0625. Available under: doi: 10.1111/exd.12155

Zusammenfassung

The neuropeptide hormone oxytocin (OXT) mediates a wide spectrum of tissue-specific actions, ranging from cell growth, cell differentiation, sodium excretion to stress responses, reproduction and complex social behaviour. Recently, OXT expression was detected in keratinocytes, but expression of its receptor and function are still unexplored in human skin. Here, we showed that both OXT and its receptor are expressed in primary human dermal fibroblasts and keratinocytes. OXT-induced dose-dependent calcium fluxes in both cell types demonstrating that the OXT receptor (OXTR) is functionally expressed. We also showed that OXT decreases proliferation of dermal fibroblasts and keratinocytes in a dose-dependent manner. In order to further investigate OXT-mediated functions in skin cells, we performed OXTR knockdown experiments. OXTR knockdown in dermal fibroblasts and keratinocytes led to elevated levels of reactive oxygen species and reduced levels of glutathione (GSH). Moreover, OXTR-depleted keratinocytes exhibited an increased release of the pro-inflammatory cytokines IL6, CCL5 and CXCL10. Our data indicate that the OXT system modulates key processes which are dysregulated in atopic dermatitis (AD) such as proliferation, inflammation and oxidative stress responses. Furthermore, we detected a downregulation of the OXT system in peri-lesional and lesional atopic skin. Taken together, these data suggest that the OXT system is a novel neuroendocrine mediator in human skin homoeostasis and clinically relevant to stressed skin conditions like AD.

Fachgebiet (DDC)

570 Biowissenschaften, Biologie

Schlagwörter

Atopic dermatitis, inflammation, oxidative stress, oxytocin system, proliferation

Zitieren

ISO 690

DEING, Verena, Dennis ROGGENKAMP, Jochen KÜHNL, Alisa GRUSCHKA, Franz STÄB, Horst WENCK, Alexander BÜRKLE, Gitta NEUFANG, 2013. Oxytocin modulates proliferation and stress responses of human skin cells : implications for atopic dermatitis. In: Experimental Dermatology. 2013, 22(6), pp. 399-405. ISSN 0906-6705. eISSN 1600-0625. Available under: doi: 10.1111/exd.12155

BibTex

@article{Deing2013-06Oxyto-25796,
  year={2013},
  doi={10.1111/exd.12155},
  title={Oxytocin modulates proliferation and stress responses of human skin cells : implications for atopic dermatitis},
  number={6},
  volume={22},
  issn={0906-6705},
  journal={Experimental Dermatology},
  pages={399--405},
  author={Deing, Verena and Roggenkamp, Dennis and Kühnl, Jochen and Gruschka, Alisa and Stäb, Franz and Wenck, Horst and Bürkle, Alexander and Neufang, Gitta}
}

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Universitätsbibliographie

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