Monitoring enzymatic ATP hydrolysis by EPR spectroscopy

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2014
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Chemical Communications. 2014, 50(55), pp. 7262-7264. ISSN 1359-7345. eISSN 1364-548X. Available under: doi: 10.1039/C4CC02422B
Zusammenfassung

An adenosine triphosphate (ATP) analogue modified with two nitroxide radicals is developed and employed to study its enzymatic hydrolysis by electron paramagnetic resonance spectroscopy. For this application, we demonstrate that EPR holds the potential to complement fluorogenic substrate analogues in monitoring enzymatic activity.

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540 Chemie
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ISO 690HACKER, Stephan M., Christian HINTZE, Andreas MARX, Malte DRESCHER, 2014. Monitoring enzymatic ATP hydrolysis by EPR spectroscopy. In: Chemical Communications. 2014, 50(55), pp. 7262-7264. ISSN 1359-7345. eISSN 1364-548X. Available under: doi: 10.1039/C4CC02422B
BibTex
@article{Hacker2014-07-14Monit-28355,
  year={2014},
  doi={10.1039/C4CC02422B},
  title={Monitoring enzymatic ATP hydrolysis by EPR spectroscopy},
  number={55},
  volume={50},
  issn={1359-7345},
  journal={Chemical Communications},
  pages={7262--7264},
  author={Hacker, Stephan M. and Hintze, Christian and Marx, Andreas and Drescher, Malte}
}
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