Efficient labelling of enzymatically synthesized vinyl-modified DNA by an inverse-electron-demand Diels-Alder reaction
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Chemical Communications. 2014, 50(74), pp. 10827-10829. ISSN 1359-7345. eISSN 1364-548X. Available under: doi: 10.1039/C4CC04332D
Zusammenfassung
Many applications in biotechnology and molecular biology rely on modified nucleotides. Here, we present an approach for the postsynthetic labelling of enzymatically synthesized vinyl-modified DNA by Diels–Alder reaction with inverse electron demand using a tetrazine. Labelling proceeds very efficiently and supersedes several known approaches.
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BUSSKAMP, Holger, Ellen BATROFF, Andrea NIEDERWIESER, Obadah S. ABDEL-RAHMAN, Rainer F. WINTER, Valentin WITTMANN, Andreas MARX, 2014. Efficient labelling of enzymatically synthesized vinyl-modified DNA by an inverse-electron-demand Diels-Alder reaction. In: Chemical Communications. 2014, 50(74), pp. 10827-10829. ISSN 1359-7345. eISSN 1364-548X. Available under: doi: 10.1039/C4CC04332DBibTex
@article{Bukamp2014-09-25Effic-28692, year={2014}, doi={10.1039/C4CC04332D}, title={Efficient labelling of enzymatically synthesized vinyl-modified DNA by an inverse-electron-demand Diels-Alder reaction}, number={74}, volume={50}, issn={1359-7345}, journal={Chemical Communications}, pages={10827--10829}, author={Bußkamp, Holger and Batroff, Ellen and Niederwieser, Andrea and Abdel-Rahman, Obadah S. and Winter, Rainer F. and Wittmann, Valentin and Marx, Andreas} }
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