Efficient labelling of enzymatically synthesized vinyl-modified DNA by an inverse-electron-demand Diels-Alder reaction

Lade...
Vorschaubild
Dateien
Busskamp_286929.pdf
Busskamp_286929.pdfGröße: 704.04 KBDownloads: 470
Datum
2014
Herausgeber:innen
Kontakt
ISSN der Zeitschrift
Electronic ISSN
ISBN
Bibliografische Daten
Verlag
Schriftenreihe
Auflagebezeichnung
DOI (zitierfähiger Link)
ArXiv-ID
Internationale Patentnummer
Angaben zur Forschungsförderung
Projekt
Open Access-Veröffentlichung
Open Access Green
Sammlungen
Core Facility der Universität Konstanz
Gesperrt bis
Titel in einer weiteren Sprache
Forschungsvorhaben
Organisationseinheiten
Zeitschriftenheft
Publikationstyp
Zeitschriftenartikel
Publikationsstatus
Published
Erschienen in
Chemical Communications. 2014, 50(74), pp. 10827-10829. ISSN 1359-7345. eISSN 1364-548X. Available under: doi: 10.1039/C4CC04332D
Zusammenfassung

Many applications in biotechnology and molecular biology rely on modified nucleotides. Here, we present an approach for the postsynthetic labelling of enzymatically synthesized vinyl-modified DNA by Diels–Alder reaction with inverse electron demand using a tetrazine. Labelling proceeds very efficiently and supersedes several known approaches.

Zusammenfassung in einer weiteren Sprache
Fachgebiet (DDC)
540 Chemie
Schlagwörter
Konferenz
Rezension
undefined / . - undefined, undefined
Zitieren
ISO 690BUSSKAMP, Holger, Ellen BATROFF, Andrea NIEDERWIESER, Obadah S. ABDEL-RAHMAN, Rainer F. WINTER, Valentin WITTMANN, Andreas MARX, 2014. Efficient labelling of enzymatically synthesized vinyl-modified DNA by an inverse-electron-demand Diels-Alder reaction. In: Chemical Communications. 2014, 50(74), pp. 10827-10829. ISSN 1359-7345. eISSN 1364-548X. Available under: doi: 10.1039/C4CC04332D
BibTex
@article{Bukamp2014-09-25Effic-28692,
  year={2014},
  doi={10.1039/C4CC04332D},
  title={Efficient labelling of enzymatically synthesized vinyl-modified DNA by an inverse-electron-demand Diels-Alder reaction},
  number={74},
  volume={50},
  issn={1359-7345},
  journal={Chemical Communications},
  pages={10827--10829},
  author={Bußkamp, Holger and Batroff, Ellen and Niederwieser, Andrea and Abdel-Rahman, Obadah S. and Winter, Rainer F. and Wittmann, Valentin and Marx, Andreas}
}
RDF
<rdf:RDF
    xmlns:dcterms="http://purl.org/dc/terms/"
    xmlns:dc="http://purl.org/dc/elements/1.1/"
    xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#"
    xmlns:bibo="http://purl.org/ontology/bibo/"
    xmlns:dspace="http://digital-repositories.org/ontologies/dspace/0.1.0#"
    xmlns:foaf="http://xmlns.com/foaf/0.1/"
    xmlns:void="http://rdfs.org/ns/void#"
    xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > 
  <rdf:Description rdf:about="https://kops.uni-konstanz.de/server/rdf/resource/123456789/28692">
    <dc:creator>Bußkamp, Holger</dc:creator>
    <bibo:uri rdf:resource="http://kops.uni-konstanz.de/handle/123456789/28692"/>
    <dcterms:bibliographicCitation>Chemical communications ; 50 (2014), 74. - S. 10827-10829</dcterms:bibliographicCitation>
    <dcterms:rights rdf:resource="https://rightsstatements.org/page/InC/1.0/"/>
    <dcterms:issued>2014-09-25</dcterms:issued>
    <dspace:hasBitstream rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/28692/1/Busskamp_286929.pdf"/>
    <dc:creator>Abdel-Rahman, Obadah S.</dc:creator>
    <dc:contributor>Niederwieser, Andrea</dc:contributor>
    <dc:contributor>Marx, Andreas</dc:contributor>
    <dc:contributor>Winter, Rainer F.</dc:contributor>
    <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2014-08-13T16:51:45Z</dc:date>
    <dc:creator>Wittmann, Valentin</dc:creator>
    <dspace:isPartOfCollection rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/29"/>
    <dcterms:isPartOf rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/29"/>
    <dcterms:hasPart rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/28692/1/Busskamp_286929.pdf"/>
    <dc:contributor>Abdel-Rahman, Obadah S.</dc:contributor>
    <dc:contributor>Bußkamp, Holger</dc:contributor>
    <dcterms:title>Efficient labelling of enzymatically synthesized vinyl-modified DNA by an inverse-electron-demand Diels-Alder reaction</dcterms:title>
    <dcterms:abstract xml:lang="eng">Many applications in biotechnology and molecular biology rely on modified nucleotides. Here, we present an approach for the postsynthetic labelling of enzymatically synthesized vinyl-modified DNA by Diels–Alder reaction with inverse electron demand using a tetrazine. Labelling proceeds very efficiently and supersedes several known approaches.</dcterms:abstract>
    <dc:creator>Winter, Rainer F.</dc:creator>
    <dc:contributor>Wittmann, Valentin</dc:contributor>
    <dc:language>eng</dc:language>
    <dc:rights>terms-of-use</dc:rights>
    <void:sparqlEndpoint rdf:resource="http://localhost/fuseki/dspace/sparql"/>
    <dc:creator>Niederwieser, Andrea</dc:creator>
    <dc:contributor>Batroff, Ellen</dc:contributor>
    <dc:creator>Marx, Andreas</dc:creator>
    <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2014-08-13T16:51:45Z</dcterms:available>
    <foaf:homepage rdf:resource="http://localhost:8080/"/>
    <dc:creator>Batroff, Ellen</dc:creator>
  </rdf:Description>
</rdf:RDF>
Interner Vermerk
xmlui.Submission.submit.DescribeStep.inputForms.label.kops_note_fromSubmitter
Kontakt
URL der Originalveröffentl.
Prüfdatum der URL
Prüfungsdatum der Dissertation
Finanzierungsart
Kommentar zur Publikation
Allianzlizenz
Corresponding Authors der Uni Konstanz vorhanden
Internationale Co-Autor:innen
Universitätsbibliographie
Ja
Begutachtet
Diese Publikation teilen