Community composition of bacterial biofilms on two submerged macrophytes and an artificial substrate in a pre-alpine lake
Dateien
Datum
Autor:innen
Herausgeber:innen
ISSN der Zeitschrift
Electronic ISSN
ISBN
Bibliografische Daten
Verlag
Schriftenreihe
Auflagebezeichnung
URI (zitierfähiger Link)
DOI (zitierfähiger Link)
Internationale Patentnummer
Link zur Lizenz
Angaben zur Forschungsförderung
Projekt
Open Access-Veröffentlichung
Sammlungen
Core Facility der Universität Konstanz
Titel in einer weiteren Sprache
Publikationstyp
Publikationsstatus
Erschienen in
Zusammenfassung
We compared the heterotrophic community composition of bacterial biofilms on the submerged macrophytes Myriophyllum spicatum and Potamogeton perfoliatus and on an artificial surface in Lower Lake Constance (Germany) on spatial (plant age) and temporal scales using denaturing gradient gel electrophoresis (DGGE) and fluorescence in situ hybridization (FISH). M. spicatum contains polyphenolic allelochemicals that inhibit algae, cyanobacteria, and heterotrophic bacteria, and possibly influence the community composition, whereas P. perfoliatus does not. In 2005, the community composition of bacterial biofilms on apices and leaves of M. spicatum differed significantly. In 2006, the biofilm communities on the apices or leaves of M. spicatum and P. perfoliatus and the artificial surface did not differ significantly, although all except one apex sample of M. spicatum formed a distinct cluster based on DGGE banding patterns. On all surfaces, members of the Cytophaga-Flavobacter-Bacteroidetes (CFB) group (16 to 22%), Alphaproteobacteria (19%), and Betaproteobacteria (7 to 31%) were abundant; Actinobacteria and Planctomycetes occurred less frequently. Sequences of DNA fragments excised from DGGE gels were mainly affiliated with yet uncultured clones originating from various freshwater habitats. Several sequences were from bacteria capable of degrading phenolic and aromatic compounds. The chemical composition of the 2 plant species and of the different parts of M. spicatum differed up to an order of magnitude. Differences in the biofilm community composition mainly depended on environmental factors (water level, conductivity, temperature, pH) and the plant chemical composition, especially the carbon and total phenolic content.
Our results suggest that the biofilm community on M. spicatum apices is related to specific bacterial functions in this microenvironment.
Zusammenfassung in einer weiteren Sprache
Fachgebiet (DDC)
Schlagwörter
Konferenz
Rezension
Zitieren
ISO 690
HEMPEL, Melanie, Hans-Peter GROSSART, Elisabeth GROSS, 2009. Community composition of bacterial biofilms on two submerged macrophytes and an artificial substrate in a pre-alpine lake. In: Aquatic Microbial Ecology. 2009, 58(1), pp. 79-94. Available under: doi: 10.3354/ame01353BibTex
@article{Hempel2009Commu-8824, year={2009}, doi={10.3354/ame01353}, title={Community composition of bacterial biofilms on two submerged macrophytes and an artificial substrate in a pre-alpine lake}, number={1}, volume={58}, journal={Aquatic Microbial Ecology}, pages={79--94}, author={Hempel, Melanie and Grossart, Hans-Peter and Gross, Elisabeth} }
RDF
<rdf:RDF xmlns:dcterms="http://purl.org/dc/terms/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#" xmlns:bibo="http://purl.org/ontology/bibo/" xmlns:dspace="http://digital-repositories.org/ontologies/dspace/0.1.0#" xmlns:foaf="http://xmlns.com/foaf/0.1/" xmlns:void="http://rdfs.org/ns/void#" xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > <rdf:Description rdf:about="https://kops.uni-konstanz.de/server/rdf/resource/123456789/8824"> <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-24T17:46:44Z</dc:date> <dcterms:rights rdf:resource="https://rightsstatements.org/page/InC/1.0/"/> <dcterms:isPartOf rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/28"/> <dcterms:title>Community composition of bacterial biofilms on two submerged macrophytes and an artificial substrate in a pre-alpine lake</dcterms:title> <dc:creator>Gross, Elisabeth</dc:creator> <dcterms:abstract xml:lang="eng">We compared the heterotrophic community composition of bacterial biofilms on the submerged macrophytes Myriophyllum spicatum and Potamogeton perfoliatus and on an artificial surface in Lower Lake Constance (Germany) on spatial (plant age) and temporal scales using denaturing gradient gel electrophoresis (DGGE) and fluorescence in situ hybridization (FISH). M. spicatum contains polyphenolic allelochemicals that inhibit algae, cyanobacteria, and heterotrophic bacteria, and possibly influence the community composition, whereas P. perfoliatus does not. In 2005, the community composition of bacterial biofilms on apices and leaves of M. spicatum differed significantly. In 2006, the biofilm communities on the apices or leaves of M. spicatum and P. perfoliatus and the artificial surface did not differ significantly, although all except one apex sample of M. spicatum formed a distinct cluster based on DGGE banding patterns. On all surfaces, members of the Cytophaga-Flavobacter-Bacteroidetes (CFB) group (16 to 22%), Alphaproteobacteria (19%), and Betaproteobacteria (7 to 31%) were abundant; Actinobacteria and Planctomycetes occurred less frequently. Sequences of DNA fragments excised from DGGE gels were mainly affiliated with yet uncultured clones originating from various freshwater habitats. Several sequences were from bacteria capable of degrading phenolic and aromatic compounds. The chemical composition of the 2 plant species and of the different parts of M. spicatum differed up to an order of magnitude. Differences in the biofilm community composition mainly depended on environmental factors (water level, conductivity, temperature, pH) and the plant chemical composition, especially the carbon and total phenolic content.<br />Our results suggest that the biofilm community on M. spicatum apices is related to specific bacterial functions in this microenvironment.</dcterms:abstract> <dc:creator>Grossart, Hans-Peter</dc:creator> <dspace:hasBitstream rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/8824/1/Hempel_etal_2009_Bacterial_biofilm_macrophytes.pdf"/> <bibo:uri rdf:resource="http://kops.uni-konstanz.de/handle/123456789/8824"/> <dc:contributor>Grossart, Hans-Peter</dc:contributor> <dc:contributor>Gross, Elisabeth</dc:contributor> <dc:creator>Hempel, Melanie</dc:creator> <dc:contributor>Hempel, Melanie</dc:contributor> <dc:rights>terms-of-use</dc:rights> <dc:language>eng</dc:language> <dcterms:issued>2009</dcterms:issued> <dcterms:bibliographicCitation>First publ. in: Aquatic Microbial Ecology 58 (2010), 1, pp. 79 94</dcterms:bibliographicCitation> <dc:format>application/pdf</dc:format> <dspace:isPartOfCollection rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/28"/> <foaf:homepage rdf:resource="http://localhost:8080/"/> <dcterms:hasPart rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/8824/1/Hempel_etal_2009_Bacterial_biofilm_macrophytes.pdf"/> <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-24T17:46:44Z</dcterms:available> <void:sparqlEndpoint rdf:resource="http://localhost/fuseki/dspace/sparql"/> </rdf:Description> </rdf:RDF>