Degradation of FAT10 by the 26S proteasome is independent of ubiquitylation but relies on NUB1L

Schmidtke, Gunter; Kalveram, Birte; Gröttrup, Marcus

Degradation of FAT10 by the 26S proteasome is independent of ubiquitylation but relies on NUB1L

Dateien

Schmidtke_opus-107543.pdfGröße: 285.59 KBDownloads: 258

Datum

2009

Autor:innen

Schmidtke, Gunter

Kalveram, Birte

Gröttrup, Marcus

Open Access-Veröffentlichung

Open Access Green

Sammlungen

Biologie

Publikationstyp

Zeitschriftenartikel

Publikationsstatus

Published

Erschienen in

FEBS Letters. 2009, 583(3), pp. 591-594. ISSN 0014-5793. eISSN 1873-3468. Available under: doi: 10.1016/j.febslet.2009.01.006

Zusammenfassung

The ubiquitin-like modifier FAT10 targets proteins for degradation by the proteasome, a process accelerated by the UBL-UBA domain protein NEDD8 ultimate buster 1-long. Here, we show that FAT10-mediated degradation occurs independently of poly-ubiquitylation as purified 26S proteasome readily degraded FAT10-dihydrofolate reductase (DHFR) but not ubiquitin-DHFR in vitro. Interestingly, the 26S proteasome could only degrade FAT10-DHFR when NUB1L was present. Knock-down of NUB1L attenuated the degradation of FAT10-DHFR in intact cells suggesting that NUB1L determines the degradation rate of FAT10-linked proteins. In conclusion, our data establish FAT10 as a ubiquitin-independent but NUB1L-dependent targeting signal for proteasomal degradation.

Fachgebiet (DDC)

570 Biowissenschaften, Biologie

Schlagwörter

Ubiquitin, FAT10, 26S proteasome, NUB1L, Degradation

Zitieren

ISO 690

SCHMIDTKE, Gunter, Birte KALVERAM, Marcus GRÖTTRUP, 2009. Degradation of FAT10 by the 26S proteasome is independent of ubiquitylation but relies on NUB1L. In: FEBS Letters. 2009, 583(3), pp. 591-594. ISSN 0014-5793. eISSN 1873-3468. Available under: doi: 10.1016/j.febslet.2009.01.006

BibTex

@article{Schmidtke2009Degra-1188,
  year={2009},
  doi={10.1016/j.febslet.2009.01.006},
  title={Degradation of FAT10 by the 26S proteasome is independent of ubiquitylation but relies on NUB1L},
  number={3},
  volume={583},
  issn={0014-5793},
  journal={FEBS Letters},
  pages={591--594},
  author={Schmidtke, Gunter and Kalveram, Birte and Gröttrup, Marcus}
}

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