A comparison of DNA and RNA quadruplex structures and stabilities

Joachimi, Astrid; Benz, Armin; Hartig, Jörg S.

A comparison of DNA and RNA quadruplex structures and stabilities

Dateien

joachimi_opus_117212.pdfGröße: 452.21 KBDownloads: 815

Datum

2009

Open Access-Veröffentlichung

Open Access Green

Sammlungen

Chemie
Zukunftskolleg

Publikationstyp

Zeitschriftenartikel

Publikationsstatus

Published

Erschienen in

Bioorganic & Medicinal Chemistry. 2009, 17(19), pp. 6811-6815. ISSN 0968-0896. eISSN 1464-3391. Available under: doi: 10.1016/j.bmc.2009.08.043

Zusammenfassung

Guanosine-rich sequences are prone to fold into four-stranded nucleic acid structures. Such quadruplex sequences have long been suspected to play important roles in regulatory processes within cells. Although DNA quadruplexes have been studied in great detail, four-stranded structures made up from RNA have received only minor attention, although it is known that RNA is able to form stable quadruplexes as well. Here, we compare quadruplex structures and stabilities of a variety of DNA and RNA sequences. We focus on well established DNA sequences and determine the topologies and stabilities of the corresponding RNA sequences by CD spectroscopy and CD thermal melting experiments. We find that the RNA sequences exclusively fold into the all-parallel conformation in contrast to the diverse topologies adopted by DNA quadruplexes. The thermal stabilities of the RNA structures rival those of the corresponding DNA sequences, often displaying enhanced stabilities compared to their DNA counterparts. Especially thermodynamically less stable sequences show a strong preference for potassium, with the RNA quadruplexes exhibiting much higher stabilities than the corresponding DNAs. The latter finding suggests that quadruplexes formed at critical positions in mRNAs might perturb gene expression to a larger extend than previously anticipated.

Fachgebiet (DDC)

540 Chemie

Zitieren

ISO 690

JOACHIMI, Astrid, Armin BENZ, Jörg S. HARTIG, 2009. A comparison of DNA and RNA quadruplex structures and stabilities. In: Bioorganic & Medicinal Chemistry. 2009, 17(19), pp. 6811-6815. ISSN 0968-0896. eISSN 1464-3391. Available under: doi: 10.1016/j.bmc.2009.08.043

BibTex

@article{Joachimi2009compa-1024,
  year={2009},
  doi={10.1016/j.bmc.2009.08.043},
  title={A comparison of DNA and RNA quadruplex structures and stabilities},
  number={19},
  volume={17},
  issn={0968-0896},
  journal={Bioorganic & Medicinal Chemistry},
  pages={6811--6815},
  author={Joachimi, Astrid and Benz, Armin and Hartig, Jörg S.}
}

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