Linking membrane microdomains to the cytoskeleton : regulation of the lateral mobility of reggie-1/flotillin-2 by interaction with actin
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The reggies/flotillins are oligomeric scaffolding proteins for membrane microdomains. We show here that reggie-1/flotillin-2 microdomains are organized along cortical F-actin in several cell types. Interaction with F-actin is mediated by the SPFH domain as shown by in vivo co-localization and in vitro binding experiments. Reggie-1/flotillin-2 microdomains form independent of actin, but disruption or stabilization of the actin cytoskeleton modulate the lateral mobility of reggie-1/flotillin-2 as shown by FRAP. Furthermore, reggie/flotillin microdomains can efficiently be immobilized by actin polymerisation, while exchange of reggie-1/flotillin-2 molecules between microdomains is enhanced by actin disruption as shown by tracking of individual microdomains using TIRF microscopy.
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LANGHORST, Matthias F., Gonzalo SOLIS PADILLA, Sylvia HANNBECK, Helmut PLATTNER, Claudia STÃœRMER, 2007. Linking membrane microdomains to the cytoskeleton : regulation of the lateral mobility of reggie-1/flotillin-2 by interaction with actin. In: FEBS Letters. 2007, 581(24), pp. 4704-4710. ISSN 0014-5793. Available under: doi: 10.1016/j.febslet.2007.08.066BibTex
@article{Langhorst2007-10-02Linki-1146, year={2007}, doi={10.1016/j.febslet.2007.08.066}, title={Linking membrane microdomains to the cytoskeleton : regulation of the lateral mobility of reggie-1/flotillin-2 by interaction with actin}, number={24}, volume={581}, issn={0014-5793}, journal={FEBS Letters}, pages={4704--4710}, author={Langhorst, Matthias F. and Solis Padilla, Gonzalo and Hannbeck, Sylvia and Plattner, Helmut and Stürmer, Claudia} }
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