Analysis of Deinococcus radiodurans mutants

Hess, Matthias

Analysis of Deinococcus radiodurans mutants

Dateien

Analysis_of_Deinococcus_radiodurans_Mutants.pdfGröße: 907.32 KBDownloads: 513

Datum

2003

Autor:innen

Hess, Matthias

URI (zitierfähiger Link)

http://nbn-resolving.de/urn:nbn:de:bsz:352-opus-12272

Link zur Lizenz

Urheberrechtlich geschützt

Open Access-Veröffentlichung

Open Access Green

Sammlungen

Biologie

Publikationstyp

Masterarbeit/Diplomarbeit

Publikationsstatus

Published

Zusammenfassung

The extremely radiation resistant vegetative bacterium Deinococcus radiodurans was isolated in 1956 from gamma-irradiated meat. Since then, it has been the subject of research aimed at understanding the genetic mechanisms underlying its resistance phenotype. In addition to being able to survive high doses of acute gamma radiation, the organism can grow under chronic gamma radiation and recover from a range of other DNA damaging conditions including exposure to desiccation, ultraviolet (UV) radiation and chemical genotoxic agents. This remarkable range of resistance has been attributed to efficient DNA repair processes capable of mending hundreds of single- and double-stranded DNA breaks without lethality or induced mutation. These characteristics were the impetus for sequencing the D. radiodurans genome, ongoing development for bioremediation, proteome and whole genome expression analyses. This thesis examines the relationships between radiation resistance, desiccation resistance, and thermotolerance in three novel D. radiodurans mutants (DR0070, DR0105, and DR2339). Mutants were tested for their ability to survive gamma radiation, desiccation, and elevated temperature. Results support that i) radiation resistance mechanisms responsible for survival following exposure to acute radiation are distinct from those needed for survival under chronic radiation, and ii) radiation resistance mechanisms responsible for survival under chronic radiation appear to be related to those involved with growth at elevated temperatures and desiccation resistance.

Fachgebiet (DDC)

570 Biowissenschaften, Biologie

Schlagwörter

Deinococcus radiodurans, recovery from DNA damage, bioremediation, radiation resistance, desiccation resistance and thermotolerance

Zitieren

ISO 690

HESS, Matthias, 2003. Analysis of Deinococcus radiodurans mutants [Master thesis]

BibTex

@mastersthesis{Hess2003Analy-8557,
  year={2003},
  title={Analysis of Deinococcus radiodurans mutants},
  author={Hess, Matthias}
}

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