Synthesis of Defined Ubiquitin Dimers

Eger, Silvia; Scheffner, Martin; Marx, Andreas; Rubini, Marina

Synthesis of Defined Ubiquitin Dimers

Dateien

Eger_JACS_2010.pdfGröße: 802.18 KBDownloads: 472

Datum

2010

Open Access-Veröffentlichung

Open Access Green

Sammlungen

Chemie

Publikationstyp

Zeitschriftenartikel

Publikationsstatus

Published

Erschienen in

Journal of the American Chemical Society. 2010, 132(46), pp. 16337-16339. ISSN 0002-7863. eISSN 1520-5126. Available under: doi: 10.1021/ja1072838

Zusammenfassung

Many proteins are post-translationally modified by the attachment of poly-ubiquitin (Ub) chains. Notably, the biological function of the attached Ub chain depends on the specific lysine residue used for conjugate formation. Here, we report an easy and efficient method to synthesize site-specifically linked Ub dimers by click reaction between two artificial amino acids. In fact, we were able to synthesize all seven naturally occurring Ub connectivities, providing the first example of a method that gives access to all Ub dimers. Furthermore, these synthetic Ub dimers are recognized by the natural ubiquitination machinery and are proteolytically stable, making them optimal candidates to further investigate the function of differently linked Ub chains.

Fachgebiet (DDC)

570 Biowissenschaften, Biologie

Zitieren

ISO 690

EGER, Silvia, Martin SCHEFFNER, Andreas MARX, Marina RUBINI, 2010. Synthesis of Defined Ubiquitin Dimers. In: Journal of the American Chemical Society. 2010, 132(46), pp. 16337-16339. ISSN 0002-7863. eISSN 1520-5126. Available under: doi: 10.1021/ja1072838

BibTex

@article{Eger2010Synth-9803,
  year={2010},
  doi={10.1021/ja1072838},
  title={Synthesis of Defined Ubiquitin Dimers},
  number={46},
  volume={132},
  issn={0002-7863},
  journal={Journal of the American Chemical Society},
  pages={16337--16339},
  author={Eger, Silvia and Scheffner, Martin and Marx, Andreas and Rubini, Marina}
}

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Universitätsbibliographie

Ja