Aptazyme-Mediated Regulation of 16S Ribosomal RNA

Wieland, Markus; Berschneider, Barbara; Erlacher, Matthias D.; Hartig, Jörg S.

Aptazyme-Mediated Regulation of 16S Ribosomal RNA

Dateien

1300.pdfGröße: 2.31 MBDownloads: 469

Datum

2010

Autor:innen

Wieland, Markus

Berschneider, Barbara

Erlacher, Matthias D.

Hartig, Jörg S.

Open Access-Veröffentlichung

Open Access Green

Sammlungen

Chemie
Zukunftskolleg

Publikationstyp

Zeitschriftenartikel

Publikationsstatus

Published

Erschienen in

Chemistry & Biology. 2010, 17(3), pp. 236-242. ISSN 1074-5521. eISSN 1879-1301. Available under: doi: 10.1016/j.chembiol.2010.02.012

Zusammenfassung

Developing artificial genetic switches in order to control gene expression via an external stimulus is an important aim in chemical and synthetic biology. Here, we expand the application range of RNA switches to the regulation of 16S rRNA function in Escherichia coli. For this purpose, we incorporated hammerhead ribozymes at several positions into orthogonalized 16S rRNA. We observed that ribosomal function is remarkably tolerant toward the incorporation of large additional RNA fragments at certain sites of the 16S rRNA. However, ribozyme-mediated cleavage results in severe reduction of 16S rRNA stability. We carried out an in vivo screen for the identification of sequences acting as ligand-responsive RNA switches, enabling thiamine-dependent switching of 16S rRNA function. In addition to expanding the regulatory toolbox, the presented artificial riboswitches should prove valuable to study aspects of rRNA folding and stability in bacteria.

Fachgebiet (DDC)

540 Chemie

Schlagwörter

CHEMBIO, RNA

Zitieren

ISO 690

WIELAND, Markus, Barbara BERSCHNEIDER, Matthias D. ERLACHER, Jörg S. HARTIG, 2010. Aptazyme-Mediated Regulation of 16S Ribosomal RNA. In: Chemistry & Biology. 2010, 17(3), pp. 236-242. ISSN 1074-5521. eISSN 1879-1301. Available under: doi: 10.1016/j.chembiol.2010.02.012

BibTex

@article{Wieland2010Aptaz-9945,
  year={2010},
  doi={10.1016/j.chembiol.2010.02.012},
  title={Aptazyme-Mediated Regulation of 16S Ribosomal RNA},
  number={3},
  volume={17},
  issn={1074-5521},
  journal={Chemistry & Biology},
  pages={236--242},
  author={Wieland, Markus and Berschneider, Barbara and Erlacher, Matthias D. and Hartig, Jörg S.}
}

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