Substrates for investigation of DNA polymerase function : synthesis and properties of 4'-C-alkylated oligonucleotides

Lade...
Vorschaubild
Dateien
Summerer.pdf
Summerer.pdfGröße: 36.1 MBDownloads: 436
Datum
2003
Autor:innen
Herausgeber:innen
Kontakt
ISSN der Zeitschrift
Electronic ISSN
ISBN
Bibliografische Daten
Verlag
Schriftenreihe
Auflagebezeichnung
DOI (zitierfähiger Link)
ArXiv-ID
Internationale Patentnummer
Angaben zur Forschungsförderung
Projekt
Open Access-Veröffentlichung
Open Access Green
Sammlungen
Core Facility der Universität Konstanz
Gesperrt bis
Titel in einer weiteren Sprache
Forschungsvorhaben
Organisationseinheiten
Zeitschriftenheft
Publikationstyp
Zeitschriftenartikel
Publikationsstatus
Published
Erschienen in
European journal of organic chemistry. 2003, 2003(10), pp. 1837-1846. ISSN 1434-193X. eISSN 1099-0690. Available under: doi: 10.1002/ejoc.200200641
Zusammenfassung

In addition to their potential as diagnostic and therapeutic agents, modified oligonucleotides have also been shown to be highly valuable tools for examination of complex biological processes. Carefully designed nucleotide analogues have therefore found considerable application in investigations of DNA polymerase function and mechanism. To examine the contribution of primarily steric constraints on DNA polymerase selectivity, we have developed a new functional strategy based on the use of modified nucleotide analogues that differ primarily in their steric demand. Here we report the efficient synthesis of modified thymidine analogues bearing 4'-alkyl groups with varying steric demand, the effects of 4'-alkylation on sugar puckering, and the incorporation of these analogues into oligonucleotides by use of automated solid-phase DNA synthesis. We also studied the pairing properties of 4'-alkylated oligonucleotide duplexes in relation to those of their natural counterparts under a range of buffer conditions. In general, our studies indicate that 4'-alkylation of thymidines has little effect on nucleoside and oligonucleotide conformation. These results have relevance to the previously reported action of 4'-alkylated nucleotides and oligonucleotides as probes of DNA polymerase function and mechanism.

Zusammenfassung in einer weiteren Sprache
Fachgebiet (DDC)
540 Chemie
Schlagwörter
Konferenz
Rezension
undefined / . - undefined, undefined
Zitieren
ISO 690DETMER, Ilka, Daniel SUMMERER, Andreas MARX, 2003. Substrates for investigation of DNA polymerase function : synthesis and properties of 4'-C-alkylated oligonucleotides. In: European journal of organic chemistry. 2003, 2003(10), pp. 1837-1846. ISSN 1434-193X. eISSN 1099-0690. Available under: doi: 10.1002/ejoc.200200641
BibTex
@article{Detmer2003Subst-478,
  year={2003},
  doi={10.1002/ejoc.200200641},
  title={Substrates for investigation of DNA polymerase function : synthesis and properties of 4'-C-alkylated oligonucleotides},
  number={10},
  volume={2003},
  issn={1434-193X},
  journal={European journal of organic chemistry},
  pages={1837--1846},
  author={Detmer, Ilka and Summerer, Daniel and Marx, Andreas}
}
RDF
<rdf:RDF
    xmlns:dcterms="http://purl.org/dc/terms/"
    xmlns:dc="http://purl.org/dc/elements/1.1/"
    xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#"
    xmlns:bibo="http://purl.org/ontology/bibo/"
    xmlns:dspace="http://digital-repositories.org/ontologies/dspace/0.1.0#"
    xmlns:foaf="http://xmlns.com/foaf/0.1/"
    xmlns:void="http://rdfs.org/ns/void#"
    xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > 
  <rdf:Description rdf:about="https://kops.uni-konstanz.de/server/rdf/resource/123456789/478">
    <dspace:isPartOfCollection rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/29"/>
    <dc:creator>Summerer, Daniel</dc:creator>
    <dc:contributor>Summerer, Daniel</dc:contributor>
    <dc:creator>Detmer, Ilka</dc:creator>
    <void:sparqlEndpoint rdf:resource="http://localhost/fuseki/dspace/sparql"/>
    <dcterms:bibliographicCitation>First publ. in: European journal of organic chemistry (2003), 10, pp. 1837 1846</dcterms:bibliographicCitation>
    <foaf:homepage rdf:resource="http://localhost:8080/"/>
    <dc:creator>Marx, Andreas</dc:creator>
    <dcterms:issued>2003</dcterms:issued>
    <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-05-31T12:10:42Z</dcterms:available>
    <dc:contributor>Detmer, Ilka</dc:contributor>
    <bibo:uri rdf:resource="http://kops.uni-konstanz.de/handle/123456789/478"/>
    <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-05-31T12:10:42Z</dc:date>
    <dspace:hasBitstream rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/478/1/Summerer.pdf"/>
    <dcterms:rights rdf:resource="https://rightsstatements.org/page/InC/1.0/"/>
    <dc:contributor>Marx, Andreas</dc:contributor>
    <dcterms:isPartOf rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/29"/>
    <dc:language>eng</dc:language>
    <dcterms:title>Substrates for investigation of DNA polymerase function : synthesis and properties of 4'-C-alkylated oligonucleotides</dcterms:title>
    <dcterms:abstract xml:lang="eng">In addition to their potential as diagnostic and therapeutic agents, modified oligonucleotides have also been shown to be highly valuable tools for examination of complex biological processes. Carefully designed nucleotide analogues have therefore found considerable application in investigations of DNA polymerase function and mechanism. To examine the contribution of primarily steric constraints on DNA polymerase selectivity, we have developed a new functional strategy based on the use of modified nucleotide analogues that differ primarily in their steric demand. Here we report the efficient synthesis of modified thymidine analogues bearing 4'-alkyl groups with varying steric demand, the effects of 4'-alkylation on sugar puckering, and the incorporation of these analogues into oligonucleotides by use of automated solid-phase DNA synthesis. We also studied the pairing properties of 4'-alkylated oligonucleotide duplexes in relation to those of their natural counterparts under a range of buffer conditions. In general, our studies indicate that 4'-alkylation of thymidines has little effect on nucleoside and oligonucleotide conformation. These results have relevance to the previously reported action of 4'-alkylated nucleotides and oligonucleotides as probes of DNA polymerase function and mechanism.</dcterms:abstract>
    <dc:rights>terms-of-use</dc:rights>
    <dcterms:hasPart rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/478/1/Summerer.pdf"/>
    <dc:format>application/pdf</dc:format>
  </rdf:Description>
</rdf:RDF>
Interner Vermerk
xmlui.Submission.submit.DescribeStep.inputForms.label.kops_note_fromSubmitter
Kontakt
URL der Originalveröffentl.
Prüfdatum der URL
Prüfungsdatum der Dissertation
Finanzierungsart
Kommentar zur Publikation
Allianzlizenz
Corresponding Authors der Uni Konstanz vorhanden
Internationale Co-Autor:innen
Universitätsbibliographie
Nein
Begutachtet
Diese Publikation teilen