@article{Heussner2010Devel-7569,
  year={2010},
  doi={10.3390/toxins2061582},
  title={Development and Characterization of a Monoclonal Antibody against Ochratoxin B and Its Application in ELISA},
  number={6},
  volume={2},
  journal={Toxins},
  pages={1582--1594},
  author={Heussner, Alexandra H. and Ausländer, Simon and Dietrich, Daniel R.}
}

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    <dcterms:bibliographicCitation>First publ. in: Toxins 2 (2010), 6, pp. 1582-1594</dcterms:bibliographicCitation>
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    <dc:contributor>Dietrich, Daniel R.</dc:contributor>
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    <dc:contributor>Ausländer, Simon</dc:contributor>
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    <dcterms:abstract xml:lang="eng">A monoclonal antibody specific to ochratoxin B (OTB) was employed for the development of an indirect competitive OTB-ELISA. The optimized OTB-ELISA resulted in a limit of detection (LOD) for OTB of 3 μg/L (8 nM), a limit of quantification (LOQ) of 3.7 μg/L (10 nM), and a 50% inhibitory concentration (IC50) of 150 nM. Due to very low cross-reactivity to OTA (2.7%) and structurally related molecules (0%), this OTB-ELISA was found to be suitable to detect OTB with excellent precision in different matrices, i.e., beer, coffee and wine. Therefore, this OTB-ELISA will allow screening of OTB in food and feed products.</dcterms:abstract>
    <dc:creator>Heussner, Alexandra H.</dc:creator>
    <dcterms:title>Development and Characterization of a Monoclonal Antibody against Ochratoxin B and Its Application in ELISA</dcterms:title>
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