Development and Characterization of a Monoclonal Antibody against Ochratoxin B and Its Application in ELISA
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2010
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Toxins. 2010, 2(6), pp. 1582-1594. eISSN 2072-6651. Available under: doi: 10.3390/toxins2061582
Zusammenfassung
A monoclonal antibody specific to ochratoxin B (OTB) was employed for the development of an indirect competitive OTB-ELISA. The optimized OTB-ELISA resulted in a limit of detection (LOD) for OTB of 3 μg/L (8 nM), a limit of quantification (LOQ) of 3.7 μg/L (10 nM), and a 50% inhibitory concentration (IC50) of 150 nM. Due to very low cross-reactivity to OTA (2.7%) and structurally related molecules (0%), this OTB-ELISA was found to be suitable to detect OTB with excellent precision in different matrices, i.e., beer, coffee and wine. Therefore, this OTB-ELISA will allow screening of OTB in food and feed products.
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Fachgebiet (DDC)
570 Biowissenschaften, Biologie
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mycotoxin, ochratoxin B, monoclonal antibody, ELISA, western analysis
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HEUSSNER, Alexandra H., Simon AUSLÄNDER, Daniel R. DIETRICH, 2010. Development and Characterization of a Monoclonal Antibody against Ochratoxin B and Its Application in ELISA. In: Toxins. 2010, 2(6), pp. 1582-1594. eISSN 2072-6651. Available under: doi: 10.3390/toxins2061582BibTex
@article{Heussner2010Devel-7569, year={2010}, doi={10.3390/toxins2061582}, title={Development and Characterization of a Monoclonal Antibody against Ochratoxin B and Its Application in ELISA}, number={6}, volume={2}, journal={Toxins}, pages={1582--1594}, author={Heussner, Alexandra H. and Ausländer, Simon and Dietrich, Daniel R.} }
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