Fermentation of methoxyacetate to glycolate and acetate by newly isolated strains of Acetobacterium sp.

Schuppert, Beate; Schink, Bernhard

Fermentation of methoxyacetate to glycolate and acetate by newly isolated strains of Acetobacterium sp.

Dateien

Fermentation_of_methoxyacetate_to_glycolate_and_acetate_by_newly_isolated_strains_of_acetobacterium_sp_1990.pdfGröße: 849.41 KBDownloads: 399

Datum

1990

Autor:innen

Schuppert, Beate

Schink, Bernhard

Open Access-Veröffentlichung

Open Access Green

Sammlungen

Biologie

Publikationstyp

Zeitschriftenartikel

Publikationsstatus

Published

Erschienen in

Archives of Microbiology. 1990, 153(2), pp. 200-204. ISSN 0302-8933. eISSN 1432-072X. Available under: doi: 10.1007/BF00247821

Zusammenfassung

Three strains of new mesophilic homoacetogenic bacteria were enriched and isolated from sewage sludge and from marine sediment samples with methoxyacetate as sole organic substrate in a carbonate-buffered medium under anoxic conditions. Two freshwater isolates were motile, Gram-positive, non-sporefonning rods. The marine strain was an immotile, Gram-positive rod with a slime capsula. All strains utilized only the methyl residue ofmethoxyacetate and released glycolic acid. They also fermented methyl groups of methoxylated aromatic compounds and of betaine to acetate with growth yields of 6-10 g dry matter per mol methyl group. H2/CO2, formate, methanol, hexamethylene tetramine, as well as fructose, numerous organic acids, glycerol, ethylene glycol, and glycol ethers were fermented to acetate as well. High activities of carbon monoxide ehydrogenase (0.4- 2.2 U x mg protein - 1) were detected in all three isolates. The guanine-plus-cytosine-content of the DNA of the freshwater isolates was 42.7 and 44.4 mol %, with the marine isolate it was 47.7 mol %. The freshwater strains were assigned to the genus Acetobacterium as new strains of the species A. carbinolicum. One freshwater isolate, strain KoMacl, was deposited with the Deutsche Sammlung von Mikroorganismen GmbH, Braunschweig, under the number DSM 5193.

Fachgebiet (DDC)

570 Biowissenschaften, Biologie

Schlagwörter

Anaerobic ether cleavage, Methoxyacetate, 2-Methoxyethanol, Demethylation, Homoacetogenic bacteria, Acetobacterium sp.

Zitieren

ISO 690

SCHUPPERT, Beate, Bernhard SCHINK, 1990. Fermentation of methoxyacetate to glycolate and acetate by newly isolated strains of Acetobacterium sp.. In: Archives of Microbiology. 1990, 153(2), pp. 200-204. ISSN 0302-8933. eISSN 1432-072X. Available under: doi: 10.1007/BF00247821

BibTex

@article{Schuppert1990Ferme-8699,
  year={1990},
  doi={10.1007/BF00247821},
  title={Fermentation of methoxyacetate to glycolate and acetate by newly isolated strains of Acetobacterium sp.},
  number={2},
  volume={153},
  issn={0302-8933},
  journal={Archives of Microbiology},
  pages={200--204},
  author={Schuppert, Beate and Schink, Bernhard}
}

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Universitätsbibliographie

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