Energy conservation in malolactic fermentation by Lactobacillus plantarum and Lactobacillus sake

Kolb, Sylvie; Otte, Hubert; Nagel, Barbara; Schink, Bernhard

Energy conservation in malolactic fermentation by Lactobacillus plantarum and Lactobacillus sake

Dateien

Energy_conservation_in_malolactic_fermentation_1992.pdfGröße: 1.43 MBDownloads: 686

Datum

1992

Autor:innen

Kolb, Sylvie

Otte, Hubert

Nagel, Barbara

Schink, Bernhard

Open Access-Veröffentlichung

Open Access Green

Sammlungen

Biologie

Publikationstyp

Zeitschriftenartikel

Publikationsstatus

Published

Erschienen in

Archives of Microbiology. 1992, 157(5), pp. 457-463. ISSN 0302-8933. eISSN 1432-072X. Available under: doi: 10.1007/BF00249105

Zusammenfassung

A comparably poor growth medium containing 0.1% yeast extract as sole non-defined constituent was developed which allowed good reproducible growth of lactic acid bacteria. Of seven different strains of lactic acid bacteria tested, only Lactobacillus plantarurn and Lactobacillus sake were found to catalyze stoichiometric conversion of L-malate to L-lactate and CO 2 concomitant with growth. The specific growth yield of malate fermentation to lactate at pH 5.0 was 2.0 g and 3.7 g per mol with L. plantarum and L. sake, respectively. Growth in batch cultures depended linearly on the malate concentration provided. Malate was decarboxylated nearly exclusively by the cytoplasmically localized malo-lactic enzyme. No other C4-dicarboxylic acid-decarboxylating enzyme activity could be detected at significant activity in cell-free extracts. In pH-controlled continuous cultures, L. plantarum grew well with glucose as ubstrate, but not with malate. Addition of lactate to continuous cultures metabolizing glucose or malate decreased cell yields significantly. These results indicate that malo-lactic fermentation by these bacteria can be coupled with energy conservation, and that membrane energetization and ATP synthesis through this metabolic activity are due to malate uptake and/or lactate excretion rather than to an ion-translocating decarboxylase enzyme.

Fachgebiet (DDC)

570 Biowissenschaften, Biologie

Schlagwörter

Malo-lactic fermentation, Bioenergetics, Transport-coupled energetization, Continuous culture, Malo-lactic enzyme, Oxalo-acetate decarboxylase

Zitieren

ISO 690

KOLB, Sylvie, Hubert OTTE, Barbara NAGEL, Bernhard SCHINK, 1992. Energy conservation in malolactic fermentation by Lactobacillus plantarum and Lactobacillus sake. In: Archives of Microbiology. 1992, 157(5), pp. 457-463. ISSN 0302-8933. eISSN 1432-072X. Available under: doi: 10.1007/BF00249105

BibTex

@article{Kolb1992Energ-7486,
  year={1992},
  doi={10.1007/BF00249105},
  title={Energy conservation in malolactic fermentation by Lactobacillus plantarum and Lactobacillus sake},
  number={5},
  volume={157},
  issn={0302-8933},
  journal={Archives of Microbiology},
  pages={457--463},
  author={Kolb, Sylvie and Otte, Hubert and Nagel, Barbara and Schink, Bernhard}
}

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