Fermentative degradation of glutarate via decarboxylation by newly isolated strictly anaerobic bacteria

Matthies, Carola; Schink, Bernhard

Fermentative degradation of glutarate via decarboxylation by newly isolated strictly anaerobic bacteria

Dateien

Fermentative_degradation_of_glutarate_via_1992.pdfGröße: 1.12 MBDownloads: 603

Datum

1992

Autor:innen

Matthies, Carola

Schink, Bernhard

Open Access-Veröffentlichung

Open Access Green

Sammlungen

Biologie

Publikationstyp

Zeitschriftenartikel

Publikationsstatus

Published

Erschienen in

Archives of Microbiology. 1992, 157(3), pp. 290-296. ISSN 0302-8933. eISSN 1432-072X. Available under: doi: 10.1007/BF00245164

Zusammenfassung

Two strains of new strictly anaerobic, gramnegative bacteria were enriched and isolated from a freshwater (strain WoG13) and a saltwater (strain CuG11)anoxic sediment with glutarate as sole energy source. Strain WoG13 formed spores whereas strain CuGll did not. Both strains were rod-shaped, motile bacteria growing in carbonate-buffered, sulfide-reduced mineral medium supplemented with 2 % of rumen fluid. Both strains fermented glutarate to butyrate, isobutyrate, CO2, and small amounts of acetate. With methylsuccinate, the same products were formed, and succinate was fermented to propionate and CO2. No sugars, amino acids or other organic acids were used as substrates. Molar growth yields (Ys) were very small (0.5-0.9 g cell dry mass/mol dicarboxylate). Cells of strain WoG13 contained no cytochromes, and the DNA base ratio was 49.0 + 1.4 mol% guanine-plus-cytosine. Enzyme activities involved in glutarate degradation could be demonstrated in cell-free extracts of strain WoG13. A pathway of glutarate fermentation via decarboxylation of glutaconyl-CoA to crotonyl-CoA is suggested which forms butyrate and partly isobutyrate by subsequent isomerization.

Fachgebiet (DDC)

570 Biowissenschaften, Biologie

Schlagwörter

Dicarboxylic acids, Glutarate, Glutaconyl, CoA decarboxylase

Zitieren

ISO 690

MATTHIES, Carola, Bernhard SCHINK, 1992. Fermentative degradation of glutarate via decarboxylation by newly isolated strictly anaerobic bacteria. In: Archives of Microbiology. 1992, 157(3), pp. 290-296. ISSN 0302-8933. eISSN 1432-072X. Available under: doi: 10.1007/BF00245164

BibTex

@article{Matthies1992Ferme-8184,
  year={1992},
  doi={10.1007/BF00245164},
  title={Fermentative degradation of glutarate via decarboxylation by newly isolated strictly anaerobic bacteria},
  number={3},
  volume={157},
  issn={0302-8933},
  journal={Archives of Microbiology},
  pages={290--296},
  author={Matthies, Carola and Schink, Bernhard}
}

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