@article{Brune1992Phlor-6827,
  year={1992},
  title={Phloroglucinol pathway in the strictly anaerobic Pelobacter acidigallici : fermentation of trihydroxybenzenes to acetate via triacetic acid},
  number={5},
  volume={157},
  issn={0302-8933},
  journal={Archives of Microbiology},
  pages={417--424},
  author={Brune, Andreas and Schink, Bernhard}
}

<rdf:RDF
    xmlns:dcterms="http://purl.org/dc/terms/"
    xmlns:dc="http://purl.org/dc/elements/1.1/"
    xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#"
    xmlns:bibo="http://purl.org/ontology/bibo/"
    xmlns:dspace="http://digital-repositories.org/ontologies/dspace/0.1.0#"
    xmlns:foaf="http://xmlns.com/foaf/0.1/"
    xmlns:void="http://rdfs.org/ns/void#"
    xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > 
  <rdf:Description rdf:about="https://kops.uni-konstanz.de/server/rdf/resource/123456789/6827">
    <bibo:uri rdf:resource="http://kops.uni-konstanz.de/handle/123456789/6827"/>
    <dc:creator>Schink, Bernhard</dc:creator>
    <dcterms:rights rdf:resource="http://creativecommons.org/licenses/by-nc-nd/2.0/"/>
    <void:sparqlEndpoint rdf:resource="http://localhost/fuseki/dspace/sparql"/>
    <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-24T17:29:28Z</dc:date>
    <dc:contributor>Schink, Bernhard</dc:contributor>
    <dspace:isPartOfCollection rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/28"/>
    <dc:contributor>Brune, Andreas</dc:contributor>
    <dcterms:abstract xml:lang="eng">The strictly anaerobic, fermenting bacterium  Pelobacter acidigallici degrades several trihydroxybenzene derivatives to stoichiometric amounts of acetate. We now report on the enzymatic activities in cell extracts which are responsible for the fermentative degradation of these aromatic compounds, and postulate a novel phloroglucinol pathway involving triacetic acid as an unusual metabolic intermediate. Gallate is decarboxylated to pyrogallol by a specific, Mg 2+-dependent, soluble enzyme activity, followed by conversion of pyrogallol to phloroglucinol, involving an unusual intermolecular transhydroxylation described previously. Phloroglucinol is then reduced to dihydrophloroglucinol (5-hydroxy-1,3-cyclohexanedione) by an NADPH-dependent phloroglucinol reductase. Dihydrophloroglucinol is cleaved hydrolytically to 3-hydroxy-5-oxohexanoic acid, which is then oxidized to triacetic acid (3,5-dioxohexanoic acid) by a unique, NADP § dehydrogenase. Triacetic acid is activated by CoA transfer from acetyl-CoA, and then converted to 3 acetyl-CoA by two subsequent fl-ketothiolase reactions. ATP is generated via phosphotransacetylase and acetate kinase.</dcterms:abstract>
    <dcterms:hasPart rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/6827/1/Phloroglucinol_pathway_in_the_strictly_anaerobic_1992.pdf"/>
    <dcterms:bibliographicCitation>First publ. in: Archives of Microbiology 157 (1992), 5, pp. 417-424</dcterms:bibliographicCitation>
    <dc:creator>Brune, Andreas</dc:creator>
    <dcterms:isPartOf rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/28"/>
    <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-24T17:29:28Z</dcterms:available>
    <dc:language>eng</dc:language>
    <dcterms:title>Phloroglucinol pathway in the strictly anaerobic Pelobacter acidigallici : fermentation of trihydroxybenzenes to acetate via triacetic acid</dcterms:title>
    <foaf:homepage rdf:resource="http://localhost:8080/"/>
    <dc:rights>Attribution-NonCommercial-NoDerivs 2.0 Generic</dc:rights>
    <dspace:hasBitstream rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/6827/1/Phloroglucinol_pathway_in_the_strictly_anaerobic_1992.pdf"/>
    <dc:format>application/pdf</dc:format>
    <dcterms:issued>1992</dcterms:issued>
  </rdf:Description>
</rdf:RDF>