Evidence of reversed electron transport in syntrophic butyrate or benzoate oxidation by Syntrophomonas wolfei and Syntrophus buswellii

Wallrabenstein, Christina; Schink, Bernhard

Evidence of reversed electron transport in syntrophic butyrate or benzoate oxidation by Syntrophomonas wolfei and Syntrophus buswellii

Dateien

Evidence_of_reversed_electron_transport_in_1994.pdfGröße: 826.55 KBDownloads: 666

Datum

1994

Autor:innen

Wallrabenstein, Christina

Schink, Bernhard

Open Access-Veröffentlichung

Open Access Green

Sammlungen

Biologie

Publikationstyp

Zeitschriftenartikel

Publikationsstatus

Published

Erschienen in

Archives of Microbiology. 1994, 162(1-2), pp. 136-142. ISSN 0302-8933. eISSN 1432-072X. Available under: doi: 10.1007/BF00264387

Zusammenfassung

Syntrophomonas wolfei and Syntrophus buswellii were grown with butyrate or benzoate in a defined binary coculture with Methanospirillum hungatei. Both strains also grew independent of the partner bacteria with crotonate as substrate. Localization of enzymes involved in butyrate oxidation by S. wolfei revealed that ATP synthase, hydrogenase, and butyryl-CoA dehydrogenase were at least partially membrane-associated whereas 3-hydroxybutyryl-CoA dehydrogenase and crotonase were entirely cytoplasmic. Inhibition experiments with copper chloride indicated that hydrogenase faced the outer surface of the cytoplasmic membrane. Suspensions of butyrate- or benzoate-grown cells of either strain accumulated hydrogen during oxidation of butyrate or benzoate to a low concentration that was thermodynamically in equilibrium with calculated reaction energetics. The protonophore carbonylcyanide m-chlorophenyl-hydrazone (CCCP) and the proton-translocating ATPase inhibitor N,N'dicyclohexylcarbodiimide (DCCD) both specifically inhibited hydrogen formation from butyrate or benzoate at low concentrations, whereas hydrogen formation from crotonate was not affected. A menaquinone was extracted from cells of S. wolfei and S. buswellii grown yntrophically in a binary methanogenic culture. The results indicate that a roton-potential-driven process is involved in hydrogen release from butyrate or benzoate oxidation.

Fachgebiet (DDC)

570 Biowissenschaften, Biologie

Schlagwörter

Syntrophic oxidation, Butyrate, Benzoate H2 formation, Syntrophus buswelliius

Zitieren

ISO 690

WALLRABENSTEIN, Christina, Bernhard SCHINK, 1994. Evidence of reversed electron transport in syntrophic butyrate or benzoate oxidation by Syntrophomonas wolfei and Syntrophus buswellii. In: Archives of Microbiology. 1994, 162(1-2), pp. 136-142. ISSN 0302-8933. eISSN 1432-072X. Available under: doi: 10.1007/BF00264387

BibTex

@article{Wallrabenstein1994Evide-7434,
  year={1994},
  doi={10.1007/BF00264387},
  title={Evidence of reversed electron transport in syntrophic butyrate or benzoate oxidation by Syntrophomonas wolfei and Syntrophus buswellii},
  number={1-2},
  volume={162},
  issn={0302-8933},
  journal={Archives of Microbiology},
  pages={136--142},
  author={Wallrabenstein, Christina and Schink, Bernhard}
}

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