Degradation of and sensitivity to cholate in Pseudomonas sp. strain Chol1

Philipp, Bodo; Erdbrink, Henrike; Suter, Marc J.-F.; Schink, Bernhard

Degradation of and sensitivity to cholate in Pseudomonas sp. strain Chol1

Dateien

Degradation_of_and_sensitivity_to_cholate_in_2006.pdfGröße: 384.63 KBDownloads: 712

Datum

2006

Autor:innen

Philipp, Bodo

Erdbrink, Henrike

Suter, Marc J.-F.

Schink, Bernhard

Open Access-Veröffentlichung

Open Access Green

Sammlungen

Biologie

Publikationstyp

Zeitschriftenartikel

Publikationsstatus

Published

Erschienen in

Archives of Microbiology. 2006, 185(3), pp. 192-201. ISSN 0302-8933. eISSN 1432-072X. Available under: doi: 10.1007/s00203-006-0085-9

Zusammenfassung

A facultative anaerobic bacterium, Pseudomonas sp. strain Chol1, degrading cholate and other bile acids was isolated from soil. We investigated how strain Chol1 grew with cholate and whether growth was affected by the toxicity of this compound. Under anoxic conditions with nitrate as electron acceptor, strain Chol1 grew by transformation of cholate to 7,12-dihydroxy-1,4-androstadiene-3,17-dione (DHADD) as end product. Under oxic conditions, strain Chol1 grew by transformation of cholate to 3,7,12-trihydroxy-9,10-seco-1,3,5(10)-androstatriene-9,17-dione (THSATD), which accumulated in the culture supernatant before its further oxidation to CO2. Strain Chol1 converted DHADD into THSATD by an oxygenase-dependent reaction. Addition of cholate ( 10 mM) to cell suspensions of strain Chol1 caused a decrease of optical density and viable counts but aerobic growth with these toxic cholate concentrations was possible. Addition of CCCP or EDTA strongly increased the sensitivity of the cells to 10 mM cholate. EDTA also increased the sensitivity of the cells to DHADD and THSATD ( £ 1.7 mM). The toxicity of cholate and its degradation intermediates with a steroid structure indicates that strain Chol1 requires a strategy to minimize these toxic effects during growth with cholate. Apparently, the proton motive force and the outer membrane are necessary for protection against these toxic effects.

Fachgebiet (DDC)

570 Biowissenschaften, Biologie

Schlagwörter

Pseudomonas, Bile acids, Cholate, Degradation, Steroids, Androstandienedione, Sensitivity

Zitieren

ISO 690

PHILIPP, Bodo, Henrike ERDBRINK, Marc J.-F. SUTER, Bernhard SCHINK, 2006. Degradation of and sensitivity to cholate in Pseudomonas sp. strain Chol1. In: Archives of Microbiology. 2006, 185(3), pp. 192-201. ISSN 0302-8933. eISSN 1432-072X. Available under: doi: 10.1007/s00203-006-0085-9

BibTex

@article{Philipp2006Degra-8016,
  year={2006},
  doi={10.1007/s00203-006-0085-9},
  title={Degradation of and sensitivity to cholate in Pseudomonas sp. strain Chol1},
  number={3},
  volume={185},
  issn={0302-8933},
  journal={Archives of Microbiology},
  pages={192--201},
  author={Philipp, Bodo and Erdbrink, Henrike and Suter, Marc J.-F. and Schink, Bernhard}
}

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