Mitochondrial and nuclear rRNA based copepod phylogeny with emphasis on the Euchaetidae (Calanoida)

Braga, Elizabeth; Zardoya, Rafael; Meyer, Axel; Yen, Jeannette

Mitochondrial and nuclear rRNA based copepod phylogeny with emphasis on the Euchaetidae (Calanoida)

Dateien

Mitochondrial_and_nuclear_rRNA_based_copepod_phylogeny_with_emphasis_on_the_Euchaetidae.pdfGröße: 622.36 KBDownloads: 673

Datum

1999

Autor:innen

Braga, Elizabeth

Zardoya, Rafael

Meyer, Axel

Yen, Jeannette

Open Access-Veröffentlichung

Open Access Green

Sammlungen

Biologie

Publikationstyp

Zeitschriftenartikel

Publikationsstatus

Published

Erschienen in

Marine Biology. 1999, 133(1), pp. 79-90. ISSN 0025-3162. eISSN 1432-1793. Available under: doi: 10.1007/s002270050445

Zusammenfassung

Phylogenetic relationships within the copepod family Euchaetidae and between representatives of three copepod orders (Calanoida, Harpacticoida, and Poecilostomatoida) were investigated using partial nucleotide sequences of the mitochondrial 16S rRNA and the nuclear 28S rRNA genes. DNA isolation, polymerase chain reaction, cloning, and DNA sequencing techniques were customized for these crustaceans. Our results support the monophyly of each copepod order, but in contrast to traditional morphology-based phylogenies of copepod orders, the Poecilostomatoida are basal to the Calanoida and Harpacticoida on our DNA-based phylogenetic tree. Phylogenetic trees generated by maximum parsimony, neighbor-joining, and maximum-likelihood analyses support the classification of the genera Euchaeta and Paraeuchaeta in the family Euchaetidae; results, however, suggest that Euchaeta acuta Giesbrecht is more closely related to species of the genus Paraeuchaeta than to those of Euchaeta, although limited taxon sampling may be partially responsible for this result. Phylogenetic mapping using the most parsimonious 16S tree suggests that the morphological synapomorphies distinguishing the genus Euchaeta evolved independently twice during the history of the Euchaetidae. Further, phylogenetic mapping suggests that the most recent common ancestor of the Euchaetidae and the Aetideidae was a deep-living, vertically migrating copepod, and that a bathypelagic, vertically migrating lifestyle characteristic of Paraeuchaeta is an ancestral trait of the family Euchaetidae which was lost apomorphically by Euchaeta. The application of a molecular clock suggests that the sibling species Euchaeta rimana Bradford and Euchaeta marina (Prestandrea) diverged due to the emergence of the Panamanian land bridge.

Fachgebiet (DDC)

570 Biowissenschaften, Biologie

Zitieren

ISO 690

BRAGA, Elizabeth, Rafael ZARDOYA, Axel MEYER, Jeannette YEN, 1999. Mitochondrial and nuclear rRNA based copepod phylogeny with emphasis on the Euchaetidae (Calanoida). In: Marine Biology. 1999, 133(1), pp. 79-90. ISSN 0025-3162. eISSN 1432-1793. Available under: doi: 10.1007/s002270050445

BibTex

@article{Braga1999Mitoc-6635,
  year={1999},
  doi={10.1007/s002270050445},
  title={Mitochondrial and nuclear rRNA based copepod phylogeny with emphasis on the Euchaetidae (Calanoida)},
  number={1},
  volume={133},
  issn={0025-3162},
  journal={Marine Biology},
  pages={79--90},
  author={Braga, Elizabeth and Zardoya, Rafael and Meyer, Axel and Yen, Jeannette}
}

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