The role of haustoria in sugar supply during infection of broad bean by the rust fungus Uromyces fabae

Voegele, Ralf T.; Struck, Christine; Hahn, Matthias; Mendgen, Kurt

The role of haustoria in sugar supply during infection of broad bean by the rust fungus Uromyces fabae

Dateien

183_Voegele_2001.pdfGröße: 564.76 KBDownloads: 604

Datum

2001

Autor:innen

Voegele, Ralf T.

Struck, Christine

Hahn, Matthias

Mendgen, Kurt

Open Access-Veröffentlichung

Open Access Green

Sammlungen

Biologie

Publikationstyp

Zeitschriftenartikel

Publikationsstatus

Published

Erschienen in

Proceedings of the National Academy of Sciences of the United States of America. 2001, 98(14), pp. 8133-8138. ISSN 0027-8424. eISSN 1091-6490. Available under: doi: 10.1073/pnas.131186798

Zusammenfassung

Biotrophic plant pathogenic fungi differentiate specialized infection structures within the living cells of their host plants. These haustoria have been linked to nutrient uptake ever since their discovery. We have for the first time to our knowledge shown that the flow of sugars from the host Vicia faba to the rust fungus Uromyces fabae seems to occur largely through the haustorial complex. One of the most abundantly expressed genes in rust haustoria, the expression of which is negligible in other fungal structures, codes for a hexose transporter. Functional expression of the gene termed HXT1 in Saccharomyces cerevisiae and Xenopus laevis oocytes assigned a substrate specificity for D-glucose and D-fructose and indicated a proton symport mechanism. Abs against HXT1p exclusively labeled haustoria in immunofluorescence microscopy and the haustorial plasma membrane in electron microscopy. These results suggest that the fungus concentrates this transporter in haustoria to take advantage of a specialized compartment of the haustorial complex. The extrahaustorial matrix, delimited by the plasma membranes of both host and parasite, constitutes a newly formed apoplastic compartment with qualities distinct from those of the bulk apoplast. This organization might facilitate the competition of the parasite with natural sink organs of the host.

Fachgebiet (DDC)

570 Biowissenschaften, Biologie

Schlagwörter

haustoria, sugar supply, Vicia faba

Zitieren

ISO 690

VOEGELE, Ralf T., Christine STRUCK, Matthias HAHN, Kurt MENDGEN, 2001. The role of haustoria in sugar supply during infection of broad bean by the rust fungus Uromyces fabae. In: Proceedings of the National Academy of Sciences of the United States of America. 2001, 98(14), pp. 8133-8138. ISSN 0027-8424. eISSN 1091-6490. Available under: doi: 10.1073/pnas.131186798

BibTex

@article{Voegele2001haust-7916,
  year={2001},
  doi={10.1073/pnas.131186798},
  title={The role of haustoria in sugar supply during infection of broad bean by the rust fungus Uromyces fabae},
  number={14},
  volume={98},
  issn={0027-8424},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  pages={8133--8138},
  author={Voegele, Ralf T. and Struck, Christine and Hahn, Matthias and Mendgen, Kurt}
}

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