Microarray analysis of expressed sequence tags from haustoria of the rust fungus Uromyces fabae

Jakupovic, Mirza; Heintz, Manuel; Reichmann, Peter; Mendgen, Kurt; Hahn, Matthias

Microarray analysis of expressed sequence tags from haustoria of the rust fungus Uromyces fabae

Dateien

204_jakupovic_et_al.pdfGröße: 564.59 KBDownloads: 1207

Datum

2006

Autor:innen

Jakupovic, Mirza

Heintz, Manuel

Reichmann, Peter

Mendgen, Kurt

Hahn, Matthias

Open Access-Veröffentlichung

Open Access Green

Sammlungen

Biologie

Publikationstyp

Zeitschriftenartikel

Publikationsstatus

Published

Erschienen in

Fungal Genetics and Biology. 2006, 43(1), pp. 8-19. ISSN 1087-1845. Available under: doi: 10.1016/j.fgb.2005.09.001

Zusammenfassung

Rust fungi are plant parasites which colonise host tissue with an intercellular mycelium that forms haustoria within living plant cells. To identify genes expressed during biotrophic growth, EST sequencing was performed with a haustorium-speciWc cDNA library from Uromyces fabae. One thousand seventeen ESTs were generated, which assembled into 530 contigs. Several of the most frequently represented sequences in the EST database were identical to the in planta induced genes (PIGs) identiWed previously (Hahn, M., Mendgen, K., 1997. Characterisation of in planta-induced rust genes isolated from a haustorium-speciWc cDNA library, Mol. Plant Microbe Interact. 10, 427 437). Virus-encoded sequences were identiWed, providing evidence for two novel RNA mycoviruses in U. fabae. Microarray hybridisation revealed many cDNAs that were signiWcantly activated in rust-infected leaves compared to germinated uredospores. Very strong in planta expression was found for two PIGs encoding putative metallothioneins. Furthermore, several genes involved in ribosome biogenesis and translation, glycolysis, amino acid metabolism, stress response, and detoxiWcation showed an increased expression in the parasitic mycelium. These data indicate a strong shift in gene expression in rust fungi between germination and the biotrophic stage of development.

Fachgebiet (DDC)

570 Biowissenschaften, Biologie

Schlagwörter

Vicia faba, Broad bean rust, EST, Biotrophy, Haustorium, cDNA library

Zitieren

ISO 690

JAKUPOVIC, Mirza, Manuel HEINTZ, Peter REICHMANN, Kurt MENDGEN, Matthias HAHN, 2006. Microarray analysis of expressed sequence tags from haustoria of the rust fungus Uromyces fabae. In: Fungal Genetics and Biology. 2006, 43(1), pp. 8-19. ISSN 1087-1845. Available under: doi: 10.1016/j.fgb.2005.09.001

BibTex

@article{Jakupovic2006Micro-8265,
  year={2006},
  doi={10.1016/j.fgb.2005.09.001},
  title={Microarray analysis of expressed sequence tags from haustoria of the rust fungus Uromyces fabae},
  number={1},
  volume={43},
  issn={1087-1845},
  journal={Fungal Genetics and Biology},
  pages={8--19},
  author={Jakupovic, Mirza and Heintz, Manuel and Reichmann, Peter and Mendgen, Kurt and Hahn, Matthias}
}

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