@article{Solomon2002Lipid-7038,
  year={2002},
  doi={10.1078/0171-2985-00114},
  title={The Lipid Raft Microdomain-Associated Protein Reggie-1/Flotillin-2 is Expressed in Human B Cells and Localized at the Plasma Membrane and Centrosome in PBMCs},
  number={1},
  volume={205},
  issn={0171-2985},
  journal={Immunobiology},
  pages={108--119},
  author={Solomon, Samuel and Masilamani, Madhan and Rajendran, Lawrence and Bastmeyer, Martin and Stürmer, Claudia and Illges, Harald}
}

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    <dc:creator>Solomon, Samuel</dc:creator>
    <dcterms:issued>2002</dcterms:issued>
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    <dcterms:title>The Lipid Raft Microdomain-Associated Protein Reggie-1/Flotillin-2 is Expressed in Human B Cells and Localized at the Plasma Membrane and Centrosome in PBMCs</dcterms:title>
    <dc:contributor>Stürmer, Claudia</dc:contributor>
    <bibo:uri rdf:resource="http://kops.uni-konstanz.de/handle/123456789/7038"/>
    <dc:contributor>Bastmeyer, Martin</dc:contributor>
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    <dc:creator>Bastmeyer, Martin</dc:creator>
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    <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-24T17:31:02Z</dc:date>
    <dc:contributor>Rajendran, Lawrence</dc:contributor>
    <dc:creator>Rajendran, Lawrence</dc:creator>
    <dc:contributor>Masilamani, Madhan</dc:contributor>
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    <dc:contributor>Solomon, Samuel</dc:contributor>
    <dcterms:abstract xml:lang="eng">Reggie-1/flotillin-2 is a plasma membrane-associated cytoplasmic protein, which defines non-caveolar raft microdomains. Reggie-1/flotillin-2 is enriched in detergent insoluble (TX100) membrane fractions (DIG), co-localizes with activated GPI-linked proteins and the fyn-kinase in neurons and T cells, and thus apparently participates in the assembly of protein complexes essential for signal transduction. In T cells activated by crosslinking the GPI-linked protein Thy-1 or by crosslinking the ganglioside GM1, reggie-1/flotillin-2 co-localizes with the T cell receptor. To determine whether reggie-1/flotillin-2 is also expressed in B cells, primary B cells from human blood and cell lines representing the developmental stages of pro, pre, mature and plasma B cells were analyzed by Western blotting, RT-PCR and immunofluorescence. Here, we show that reggie-1/flotillin-2 is expressed throughout B cell development, as well as in primary B cells, purified by cell sorting. On non-activated mature B cell Raji cell line we found reggie-1/flotillin-2 are exclusively in the detergent (TX100) insoluble membrane fractions that are staining positive for the raft marker GM1. Immunofluorescence microscopy showed that reggie-1/flotillin-2 is localized at the plasma membrane and marks intracellular spots in PBMCs. Confocal co-localization studies showed that reggie-1/flotillin-2 is associated with the plasma membrane, and the centrosomes (microtubule organizing centers) in these PBMCs. Comparison of reggie-1/flotillin-2 cDNA sequences with the genomic sequence database allowed us to determine the exon/intron structures in mouse and human. The gene organizations are highly conserved suggesting an important function of reggie-1/flotillin-2. Since reggie/flotillin proteins co-cluster with the T cell receptor and fyn kinases upon T cell stimulation, our findings of reggie-1/flotillin-2 in B cells suggest a similar role in B cell function.</dcterms:abstract>
    <dc:creator>Stürmer, Claudia</dc:creator>
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    <dcterms:bibliographicCitation>First publ. in: Immunobiology 205 (2002), 1, pp. 108-119</dcterms:bibliographicCitation>
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    <dc:creator>Illges, Harald</dc:creator>
    <dc:contributor>Illges, Harald</dc:contributor>
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    <dc:language>eng</dc:language>
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