Crystal Structure of the Sugar Binding Domain of the Archaeal Transcritional Regulator TrmB

Krug, Michael; Lee, Sung-Jae; Diederichs, Kay; Boos, Winfried; Welte, Wolfram

Crystal Structure of the Sugar Binding Domain of the Archaeal Transcritional Regulator TrmB

Dateien

74_Krug_JBC_281_10976.pdfGröße: 503.69 KBDownloads: 270

Datum

2006

Autor:innen

Lee, Sung-Jae

Open Access-Veröffentlichung

Open Access Green

Sammlungen

Biologie

Publikationstyp

Zeitschriftenartikel

Publikationsstatus

Published

Erschienen in

Journal of Biological Chemistry. 2006, 281(16), pp. 10976-10982. ISSN 0021-9258. eISSN 1083-351X. Available under: doi: 10.1074/jbc.M512809200

Zusammenfassung

TrmB is an alpha-glucoside-sensing transcriptional regulator controlling two operons encoding maltose/trehalose and maltodextrin ABC transporters of Pyrococcus furiosus. The crystal structure of an N-terminal truncated derivative of TrmB was solved at 1.5A resolution. This protein has lost its DNA binding domain but has retained its sugar recognition site. The structure represents a novel sugar-binding fold. TrmB bound maltose, glucose, sucrose, and maltotriose, exhibiting Kd values of 6.8, 25, 34, and 160 μM, respectively. TrmB behaved as a monomer in dilute buffer solution in contrast to the full-length protein, which is a dimer. Co-crystallization with bound maltose identified a binding site involving seven amino acid residues: Ser229, Asn305, Gly320, Met321, Val324, Ile325, and Glu326. Six of these residues interact with the nonreducing glucosyl residue of maltose. The nonreducing glucosyl residue is shared by all substrates bound to TrmB, suggesting it as a common recognition motif.

Fachgebiet (DDC)

570 Biowissenschaften, Biologie

Zitieren

ISO 690

KRUG, Michael, Sung-Jae LEE, Kay DIEDERICHS, Winfried BOOS, Wolfram WELTE, 2006. Crystal Structure of the Sugar Binding Domain of the Archaeal Transcritional Regulator TrmB. In: Journal of Biological Chemistry. 2006, 281(16), pp. 10976-10982. ISSN 0021-9258. eISSN 1083-351X. Available under: doi: 10.1074/jbc.M512809200

BibTex

@article{Krug2006Cryst-7898,
  year={2006},
  doi={10.1074/jbc.M512809200},
  title={Crystal Structure of the Sugar Binding Domain of the Archaeal Transcritional Regulator TrmB},
  number={16},
  volume={281},
  issn={0021-9258},
  journal={Journal of Biological Chemistry},
  pages={10976--10982},
  author={Krug, Michael and Lee, Sung-Jae and Diederichs, Kay and Boos, Winfried and Welte, Wolfram}
}

RDF

<rdf:RDF
    xmlns:dcterms="http://purl.org/dc/terms/"
    xmlns:dc="http://purl.org/dc/elements/1.1/"
    xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#"
    xmlns:bibo="http://purl.org/ontology/bibo/"
    xmlns:dspace="http://digital-repositories.org/ontologies/dspace/0.1.0#"
    xmlns:foaf="http://xmlns.com/foaf/0.1/"
    xmlns:void="http://rdfs.org/ns/void#"
    xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > 
  <rdf:Description rdf:about="https://kops.uni-konstanz.de/server/rdf/resource/123456789/7898">
    <dcterms:issued>2006</dcterms:issued>
    <dc:language>eng</dc:language>
    <dcterms:bibliographicCitation>First publ. in: Journal of Biological Chemistry 281 (2006), 16, pp. 10976-10982</dcterms:bibliographicCitation>
    <dspace:hasBitstream rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/7898/1/74_Krug_JBC_281_10976.pdf"/>
    <dc:format>application/pdf</dc:format>
    <dc:rights>Attribution-NonCommercial-NoDerivs 2.0 Generic</dc:rights>
    <dcterms:title>Crystal Structure of the Sugar Binding Domain of the Archaeal Transcritional Regulator TrmB</dcterms:title>
    <dc:contributor>Welte, Wolfram</dc:contributor>
    <dc:creator>Diederichs, Kay</dc:creator>
    <dc:contributor>Krug, Michael</dc:contributor>
    <dc:creator>Lee, Sung-Jae</dc:creator>
    <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-24T17:38:22Z</dcterms:available>
    <dspace:isPartOfCollection rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/28"/>
    <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-24T17:38:22Z</dc:date>
    <dcterms:hasPart rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/7898/1/74_Krug_JBC_281_10976.pdf"/>
    <void:sparqlEndpoint rdf:resource="http://localhost/fuseki/dspace/sparql"/>
    <dcterms:abstract xml:lang="eng">TrmB is an alpha-glucoside-sensing transcriptional regulator controlling two operons encoding maltose/trehalose and maltodextrin ABC transporters of Pyrococcus furiosus. The crystal structure of an N-terminal truncated derivative of TrmB was solved at 1.5A resolution. This protein has lost its DNA binding domain but has retained its sugar recognition site. The structure represents a novel sugar-binding fold. TrmB bound maltose, glucose, sucrose, and maltotriose, exhibiting Kd values of 6.8, 25, 34, and 160 μM, respectively. TrmB behaved as a monomer in dilute buffer solution in contrast to the full-length protein, which is a dimer. Co-crystallization with bound maltose identified a binding site involving seven amino acid residues: Ser229, Asn305, Gly320, Met321, Val324, Ile325, and Glu326. Six of these residues interact with the nonreducing glucosyl residue of maltose. The nonreducing glucosyl residue is shared by all substrates bound to TrmB, suggesting it as a common recognition motif.</dcterms:abstract>
    <dc:contributor>Boos, Winfried</dc:contributor>
    <dc:creator>Krug, Michael</dc:creator>
    <dcterms:isPartOf rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/28"/>
    <dc:contributor>Diederichs, Kay</dc:contributor>
    <bibo:uri rdf:resource="http://kops.uni-konstanz.de/handle/123456789/7898"/>
    <dc:creator>Welte, Wolfram</dc:creator>
    <dc:contributor>Lee, Sung-Jae</dc:contributor>
    <foaf:homepage rdf:resource="http://localhost:8080/"/>
    <dcterms:rights rdf:resource="http://creativecommons.org/licenses/by-nc-nd/2.0/"/>
    <dc:creator>Boos, Winfried</dc:creator>
  </rdf:Description>
</rdf:RDF>

Universitätsbibliographie

Ja