Crystallization and preliminary X-ray diffraction analysis of ScrY, a specific bacterial outer membrane porin

Forst, Doris; Schülein, Katrin; Wacker, Thomas; Diederichs, Kay; Kreutz, Werner; Benz, Roland; Welte, Wolfram

Crystallization and preliminary X-ray diffraction analysis of ScrY, a specific bacterial outer membrane porin

Dateien

$Crystallization_and_preliminary_X_ray_diffraction_analysis_of_ScrY.pdf$

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Datum

1993

Autor:innen

Forst, Doris

Schülein, Katrin

Wacker, Thomas

Diederichs, Kay

Kreutz, Werner

Benz, Roland

Welte, Wolfram

Open Access-Veröffentlichung

Open Access Green

Sammlungen

Biologie

Publikationstyp

Zeitschriftenartikel

Publikationsstatus

Published

Erschienen in

Journal of Molecular Biology. 1993, 229(1), pp. 258-262. ISSN 0022-2836. Available under: doi: 10.1006/jmbi.1993.1028

Zusammenfassung

The sucrose-specific outer membrane porin ScrY of Salmonella typhimurium was isolated from Escherichia coli K-12 strain KS 26 containing the plasmid pPSO112. The protein was purified to homogeneity by differential extraction of the cell envelope in the presence of the detergents sodium dodecyl sulfate and lauryl (dimethyl)-amine oxide (LDAO). The porin had apparent molecular weights of 58 kDa and 120 kDa for the monomer and for the trimer, respectively, on SDS/PAGE. The purified trimers were crystallized using poly(ethylene glycol) 2000 and the detergents octylglucoside (OG) and hexyl-(dimethyl)-amine oxide (C6DAO). X-ray diffraction of the crystals showed reflections to 2'3 Å. The space group of the crystals was R3 and the lattice constants of the hexagonal axes were a = b = 112'85 Å and c =149'9 Å. The crystal volume per unit of protein molecular weight was 3'47 Å3/Da.

Fachgebiet (DDC)

570 Biowissenschaften, Biologie

Schlagwörter

porin, sucrose transport, membrane protein, crystallization, E. coli

Zitieren

ISO 690

FORST, Doris, Katrin SCHÜLEIN, Thomas WACKER, Kay DIEDERICHS, Werner KREUTZ, Roland BENZ, Wolfram WELTE, 1993. Crystallization and preliminary X-ray diffraction analysis of ScrY, a specific bacterial outer membrane porin. In: Journal of Molecular Biology. 1993, 229(1), pp. 258-262. ISSN 0022-2836. Available under: doi: 10.1006/jmbi.1993.1028

BibTex

@article{Forst1993Cryst-8304,
  year={1993},
  doi={10.1006/jmbi.1993.1028},
  title={Crystallization and preliminary X-ray diffraction analysis of ScrY, a specific bacterial outer membrane porin},
  number={1},
  volume={229},
  issn={0022-2836},
  journal={Journal of Molecular Biology},
  pages={258--262},
  author={Forst, Doris and Schülein, Katrin and Wacker, Thomas and Diederichs, Kay and Kreutz, Werner and Benz, Roland and Welte, Wolfram}
}

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