Crystal structure of MaIK, the ATPase subunit of the trehalose/maltose ABC transporter of the archaeon Thermococcus litoralis

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2000
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Diez, Joachim
Greller, Gerhard
Müller, Christian
Breed, Jason
Schnell, Christoph
Vonrhein, Clemens
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The EMBO Journal. 2000, 19(22), pp. 5951-5961. ISSN 1460-2075. eISSN 1460-2075. Available under: doi: 10.1093/emboj/19.22.5951
Zusammenfassung

The members of the ABC transporter family transport a wide variety of molecules into or out of cellsand cellular compartments. Apart from a translocationpore, each member possesses two similar nucleoside triphosphate-binding subunits or domains in order to couple the energy-providing reaction with transport. In the maltose transporter of several Gram-negative hacteria and the archaeon Thermococcus litoralis, the nucleoside triphosphate-binding subunit contains a C-terminal regulatory domain. A dimer of the subunit is attached cytoplasmically to the translocation pore. Here we report the crystal structure of this dimer showing two bound pyrophosphate molecules at 1.9 Å resolution. The dimer forms by association of the ATPase domains, with the two regulatory domains attached at opposite poles. Significant deviation from 2-fold symmetry is seen at the interface of the dimer and in the regions corresponding to those residues known to be in contact with the translocation pore. The structure and its relationship to function are discussed in the light of known mutations from the homologous Escherichia coli and Salmonella typhimurium proteins.

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570 Biowissenschaften, Biologie
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active transport, CFTR, crystal structure, maltose uptake and regulation, P-glycoprotein
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ISO 690DIEDERICHS, Kay, Joachim DIEZ, Gerhard GRELLER, Christian MÜLLER, Jason BREED, Christoph SCHNELL, Clemens VONRHEIN, Winfried BOOS, Wolfram WELTE, 2000. Crystal structure of MaIK, the ATPase subunit of the trehalose/maltose ABC transporter of the archaeon Thermococcus litoralis. In: The EMBO Journal. 2000, 19(22), pp. 5951-5961. ISSN 1460-2075. eISSN 1460-2075. Available under: doi: 10.1093/emboj/19.22.5951
BibTex
@article{Diederichs2000-11-15Cryst-8726,
  year={2000},
  doi={10.1093/emboj/19.22.5951},
  title={Crystal structure of MaIK, the ATPase subunit of the trehalose/maltose ABC transporter of the archaeon Thermococcus litoralis},
  number={22},
  volume={19},
  issn={1460-2075},
  journal={The EMBO Journal},
  pages={5951--5961},
  author={Diederichs, Kay and Diez, Joachim and Greller, Gerhard and Müller, Christian and Breed, Jason and Schnell, Christoph and Vonrhein, Clemens and Boos, Winfried and Welte, Wolfram}
}
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