Influence of sodium concentration on changes of membrane capacitance associated with the electrogenic ion transport by the Na,K-ATPase

Lade...
Vorschaubild
Dateien
Influence_of_sodium.pdf
Influence_of_sodium.pdfGröße: 206.44 KBDownloads: 457
Datum
1998
Autor:innen
Sokolov, Valerij S.
Stukolov, S. M.
Darmostuk, A. S.
Herausgeber:innen
Kontakt
ISSN der Zeitschrift
Electronic ISSN
ISBN
Bibliografische Daten
Verlag
Schriftenreihe
Auflagebezeichnung
ArXiv-ID
Internationale Patentnummer
Angaben zur Forschungsförderung
Projekt
Open Access-Veröffentlichung
Open Access Green
Sammlungen
Core Facility der Universität Konstanz
Gesperrt bis
Titel in einer weiteren Sprache
Forschungsvorhaben
Organisationseinheiten
Zeitschriftenheft
Publikationstyp
Zeitschriftenartikel
Publikationsstatus
Published
Erschienen in
European Biophysics Journal. 1998, 27(6), pp. 605-617. ISSN 0175-7571. eISSN 1432-1017. Available under: doi: 10.1007/s002490050172
Zusammenfassung

Electrogenic ion transport by the Na,K-ATPase was investigated in a model system of protein-containing membrane fragments adsorbed to a lipid bilayer. Transient Na+ currents were induced by photorelease of ATP from inactive caged ATP. This process was accompanied by a capacitance change of the membrane system. Two methods were applied to measure capacitances in the frequency range 1 to 6000 Hz. It was, however, dependent on Na+ concentration and on the membrane potential which was generated by the charge transferred. Below 5 mM Na+ a negative capacitance change was detected which may be assigned to electrogenic Na+ binding to cytoplasmic sites. It could be shown that the results obtained by experiments with the presented alternating current method contain the information which is determined by current-relaxation experiments with cell membranes.

Zusammenfassung in einer weiteren Sprache
Fachgebiet (DDC)
570 Biowissenschaften, Biologie
Schlagwörter
Na,K-ATPase, membrane capacitance, Dielectric properties, Ion movement, Electrogenicity
Konferenz
Rezension
undefined / . - undefined, undefined
Zitieren
ISO 690SOKOLOV, Valerij S., S. M. STUKOLOV, A. S. DARMOSTUK, Hans-Jürgen APELL, 1998. Influence of sodium concentration on changes of membrane capacitance associated with the electrogenic ion transport by the Na,K-ATPase. In: European Biophysics Journal. 1998, 27(6), pp. 605-617. ISSN 0175-7571. eISSN 1432-1017. Available under: doi: 10.1007/s002490050172
BibTex
@article{Sokolov1998Influ-8473,
  year={1998},
  doi={10.1007/s002490050172},
  title={Influence of sodium concentration on changes of membrane capacitance associated with the electrogenic ion transport by the Na,K-ATPase},
  number={6},
  volume={27},
  issn={0175-7571},
  journal={European Biophysics Journal},
  pages={605--617},
  author={Sokolov, Valerij S. and Stukolov, S. M. and Darmostuk, A. S. and Apell, Hans-Jürgen}
}
RDF
<rdf:RDF
    xmlns:dcterms="http://purl.org/dc/terms/"
    xmlns:dc="http://purl.org/dc/elements/1.1/"
    xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#"
    xmlns:bibo="http://purl.org/ontology/bibo/"
    xmlns:dspace="http://digital-repositories.org/ontologies/dspace/0.1.0#"
    xmlns:foaf="http://xmlns.com/foaf/0.1/"
    xmlns:void="http://rdfs.org/ns/void#"
    xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > 
  <rdf:Description rdf:about="https://kops.uni-konstanz.de/server/rdf/resource/123456789/8473">
    <dc:creator>Darmostuk, A. S.</dc:creator>
    <dc:language>eng</dc:language>
    <dcterms:issued>1998</dcterms:issued>
    <dcterms:abstract xml:lang="eng">Electrogenic ion transport by the Na,K-ATPase was investigated in a model system of protein-containing membrane fragments adsorbed to a lipid bilayer. Transient Na+ currents were induced by photorelease of ATP from inactive caged ATP. This process was accompanied by a capacitance change of the membrane system. Two methods were applied to measure capacitances in the frequency range 1 to 6000 Hz. It was, however, dependent on Na+ concentration and on the membrane potential which was generated by the charge transferred. Below 5 mM Na+ a negative capacitance change was detected which may be assigned to electrogenic Na+ binding to cytoplasmic sites. It could be shown that the results obtained by experiments with the presented alternating current method contain the information which is determined by current-relaxation experiments with cell membranes.</dcterms:abstract>
    <dc:contributor>Stukolov, S. M.</dc:contributor>
    <dc:format>application/pdf</dc:format>
    <dspace:hasBitstream rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/8473/1/Influence_of_sodium.pdf"/>
    <dc:contributor>Sokolov, Valerij S.</dc:contributor>
    <dcterms:bibliographicCitation>First publ. in: European Biophysics Journal 27 (1998), pp. 605-617</dcterms:bibliographicCitation>
    <dc:creator>Sokolov, Valerij S.</dc:creator>
    <dcterms:rights rdf:resource="http://creativecommons.org/licenses/by-nc-nd/2.0/"/>
    <dcterms:title>Influence of sodium concentration on changes of membrane capacitance associated with the electrogenic ion transport by the Na,K-ATPase</dcterms:title>
    <dc:contributor>Darmostuk, A. S.</dc:contributor>
    <dcterms:hasPart rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/8473/1/Influence_of_sodium.pdf"/>
    <dc:rights>Attribution-NonCommercial-NoDerivs 2.0 Generic</dc:rights>
    <dspace:isPartOfCollection rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/28"/>
    <foaf:homepage rdf:resource="http://localhost:8080/"/>
    <dc:creator>Stukolov, S. M.</dc:creator>
    <dc:contributor>Apell, Hans-Jürgen</dc:contributor>
    <dc:creator>Apell, Hans-Jürgen</dc:creator>
    <bibo:uri rdf:resource="http://kops.uni-konstanz.de/handle/123456789/8473"/>
    <void:sparqlEndpoint rdf:resource="http://localhost/fuseki/dspace/sparql"/>
    <dcterms:isPartOf rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/28"/>
    <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-24T17:43:56Z</dc:date>
    <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-24T17:43:56Z</dcterms:available>
  </rdf:Description>
</rdf:RDF>
Interner Vermerk
xmlui.Submission.submit.DescribeStep.inputForms.label.kops_note_fromSubmitter
Kontakt
URL der Originalveröffentl.
Prüfdatum der URL
Prüfungsdatum der Dissertation
Finanzierungsart
Kommentar zur Publikation
Allianzlizenz
Corresponding Authors der Uni Konstanz vorhanden
Internationale Co-Autor:innen
Universitätsbibliographie
Ja
Begutachtet
Diese Publikation teilen