FAK integrates growth-factor and integrin signals to promote cell migration

Sieg, David J.; Hauck, Christof R.; Ilić, Du ko; Klingbeil, Candice K.; Schaefer, Erik; Damsky, Caroline H.; Schlaepfer, David D.

FAK integrates growth-factor and integrin signals to promote cell migration

Dateien

FAK_integrates_growth_factor_and_integrin_signals_to_promote_cell_migration.pdfGröße: 446.27 KBDownloads: 2578

Datum

2000

Autor:innen

Sieg, David J.

Hauck, Christof R.

Ilić, Du ko

Klingbeil, Candice K.

Schaefer, Erik

Damsky, Caroline H.

Schlaepfer, David D.

Open Access-Veröffentlichung

Open Access Green

Sammlungen

Biologie

Publikationstyp

Zeitschriftenartikel

Publikationsstatus

Published

Erschienen in

Nature Cell Biology. 2000, 2(5), pp. 249-257. ISSN 1465-7392. Available under: doi: 10.1038/35010517

Zusammenfassung

Here we show that cells lacking focal adhesion kinase (FAK) are refractory to motility signals from platelet-derived and epidermal growth factors (PDGF and EGF respectively), and that stable re-expression of FAK rescues these defects. FAK associates with activated PDGF- and EGF-receptor (PDGFR and EGFR) signalling complexes, and expression of the band-4.1-like domain at the FAK amino terminus is sufficient to mediate an interaction with activated EGFR. However, efficient EGF-stimulated cell migration also requires FAK to be targeted, by its carboxy-terminal domain, to sites of integrin-receptor clustering. Although the kinase activity of FAK is not needed to promote PDGF- or EGF-stimulated cell motility, kinase-inactive FAK is transphosphorylated at the indispensable Src-kinase-binding site, FAK Y397, after EGF stimulation of cells. Our results establish that FAK is an important receptor-proximal link between growth-factor-receptor and integrin signalling pathways.

Fachgebiet (DDC)

570 Biowissenschaften, Biologie

Zitieren

ISO 690

SIEG, David J., Christof R. HAUCK, Du ko ILIĆ, Candice K. KLINGBEIL, Erik SCHAEFER, Caroline H. DAMSKY, David D. SCHLAEPFER, 2000. FAK integrates growth-factor and integrin signals to promote cell migration. In: Nature Cell Biology. 2000, 2(5), pp. 249-257. ISSN 1465-7392. Available under: doi: 10.1038/35010517

BibTex

@article{Sieg2000integ-8279,
  year={2000},
  doi={10.1038/35010517},
  title={FAK integrates growth-factor and integrin signals to promote cell migration},
  number={5},
  volume={2},
  issn={1465-7392},
  journal={Nature Cell Biology},
  pages={249--257},
  author={Sieg, David J. and Hauck, Christof R. and Ilić, Du ko and Klingbeil, Candice K. and Schaefer, Erik and Damsky, Caroline H. and Schlaepfer, David D.}
}

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Universitätsbibliographie

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