Binding of the third Na+ ion to the cytoplasmatic side of the Na,K-ATPase is electrogenic

Lade...
Vorschaubild
Dateien
Binding_of_the_third.pdf
Binding_of_the_third.pdfGröße: 234.45 KBDownloads: 407
Datum
1999
Autor:innen
Domaszewicz, Wolfgang
Herausgeber:innen
Kontakt
ISSN der Zeitschrift
Electronic ISSN
ISBN
Bibliografische Daten
Verlag
Schriftenreihe
Auflagebezeichnung
ArXiv-ID
Internationale Patentnummer
Angaben zur Forschungsförderung
Projekt
Open Access-Veröffentlichung
Open Access Green
Sammlungen
Core Facility der Universität Konstanz
Gesperrt bis
Titel in einer weiteren Sprache
Forschungsvorhaben
Organisationseinheiten
Zeitschriftenheft
Publikationstyp
Zeitschriftenartikel
Publikationsstatus
Published
Erschienen in
FEBS Letters. 1999, 458(2), pp. 241-246. ISSN 0014-5793. Available under: doi: 10.1016/S0014-5793(99)01162-X
Zusammenfassung

A new experimental setup was constructed to allow parallel measurements of total internal reflection fluorescence and of capacitance changes in Na,K-ATPase-containing membranes. Effects correlated with cytoplasmatic sodium binding to Na,K-ATPase were investigated. Ion binding-induced fluorescence changes of the electrochromic dye RH421 in membrane fragments adsorbed on a transparent capacitative electrode corresponded perfectly to capacitance increases detected by a lock-in technique. From these electric measurements it was possible to estimate a dielectric coefficient of about 0.25 for the electrogenetic binding of the third Na+ ion. Binding of K+ to cytoplasmic sites was electroneutral.

Zusammenfassung in einer weiteren Sprache
Fachgebiet (DDC)
570 Biowissenschaften, Biologie
Schlagwörter
Sodium pump, Ion movement, Electrogenic binding, Fluorescent dye, Capacitative coupling
Konferenz
Rezension
undefined / . - undefined, undefined
Zitieren
ISO 690DOMASZEWICZ, Wolfgang, Hans-Jürgen APELL, 1999. Binding of the third Na+ ion to the cytoplasmatic side of the Na,K-ATPase is electrogenic. In: FEBS Letters. 1999, 458(2), pp. 241-246. ISSN 0014-5793. Available under: doi: 10.1016/S0014-5793(99)01162-X
BibTex
@article{Domaszewicz1999Bindi-7852,
  year={1999},
  doi={10.1016/S0014-5793(99)01162-X},
  title={Binding of the third Na+ ion to the cytoplasmatic side of the Na,K-ATPase is electrogenic},
  number={2},
  volume={458},
  issn={0014-5793},
  journal={FEBS Letters},
  pages={241--246},
  author={Domaszewicz, Wolfgang and Apell, Hans-Jürgen}
}
RDF
<rdf:RDF
    xmlns:dcterms="http://purl.org/dc/terms/"
    xmlns:dc="http://purl.org/dc/elements/1.1/"
    xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#"
    xmlns:bibo="http://purl.org/ontology/bibo/"
    xmlns:dspace="http://digital-repositories.org/ontologies/dspace/0.1.0#"
    xmlns:foaf="http://xmlns.com/foaf/0.1/"
    xmlns:void="http://rdfs.org/ns/void#"
    xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > 
  <rdf:Description rdf:about="https://kops.uni-konstanz.de/server/rdf/resource/123456789/7852">
    <dc:creator>Domaszewicz, Wolfgang</dc:creator>
    <bibo:uri rdf:resource="http://kops.uni-konstanz.de/handle/123456789/7852"/>
    <dc:contributor>Domaszewicz, Wolfgang</dc:contributor>
    <dc:language>eng</dc:language>
    <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-24T17:38:01Z</dcterms:available>
    <foaf:homepage rdf:resource="http://localhost:8080/"/>
    <dc:contributor>Apell, Hans-Jürgen</dc:contributor>
    <void:sparqlEndpoint rdf:resource="http://localhost/fuseki/dspace/sparql"/>
    <dcterms:bibliographicCitation>First publ. in: FEBS Letters 458 (1999), pp. 241-246</dcterms:bibliographicCitation>
    <dcterms:rights rdf:resource="http://creativecommons.org/licenses/by-nc-nd/2.0/"/>
    <dc:creator>Apell, Hans-Jürgen</dc:creator>
    <dc:rights>Attribution-NonCommercial-NoDerivs 2.0 Generic</dc:rights>
    <dcterms:issued>1999</dcterms:issued>
    <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-24T17:38:01Z</dc:date>
    <dcterms:abstract xml:lang="eng">A new experimental setup was constructed to allow parallel measurements of total internal reflection fluorescence and of capacitance changes in Na,K-ATPase-containing membranes. Effects correlated with cytoplasmatic sodium binding to Na,K-ATPase were investigated. Ion binding-induced fluorescence changes of the electrochromic dye RH421 in membrane fragments adsorbed on a transparent capacitative electrode corresponded perfectly to capacitance increases detected by a lock-in technique. From these electric measurements it was possible to estimate a dielectric coefficient of about 0.25 for the electrogenetic binding of the third Na+ ion. Binding of K+ to cytoplasmic sites was electroneutral.</dcterms:abstract>
    <dspace:isPartOfCollection rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/28"/>
    <dspace:hasBitstream rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/7852/1/Binding_of_the_third.pdf"/>
    <dcterms:title>Binding of the third Na+ ion to the cytoplasmatic side of the Na,K-ATPase is electrogenic</dcterms:title>
    <dc:format>application/pdf</dc:format>
    <dcterms:isPartOf rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/28"/>
    <dcterms:hasPart rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/7852/1/Binding_of_the_third.pdf"/>
  </rdf:Description>
</rdf:RDF>
Interner Vermerk
xmlui.Submission.submit.DescribeStep.inputForms.label.kops_note_fromSubmitter
Kontakt
URL der Originalveröffentl.
Prüfdatum der URL
Prüfungsdatum der Dissertation
Finanzierungsart
Kommentar zur Publikation
Allianzlizenz
Corresponding Authors der Uni Konstanz vorhanden
Internationale Co-Autor:innen
Universitätsbibliographie
Ja
Begutachtet
Diese Publikation teilen