@article{Sehring2004oscil-6607,
  year={2004},
  title={Ca2+ oscillations mediated by exogenous GTP in Paramecium cells : assessment of possible Ca2+ sources},
  number={5},
  volume={36},
  issn={0143-4160},
  journal={Cell Calcium},
  pages={409--420},
  author={Sehring, Ivonne Margarete and Plattner, Helmut}
}

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    <dcterms:bibliographicCitation>First publ. in: Cell Calcium 36 (2004), pp. 409-420</dcterms:bibliographicCitation>
    <dcterms:title>Ca2+ oscillations mediated by exogenous GTP in Paramecium cells : assessment of possible Ca2+ sources</dcterms:title>
    <dc:creator>Sehring, Ivonne Margarete</dc:creator>
    <dc:contributor>Sehring, Ivonne Margarete</dc:contributor>
    <dcterms:abstract xml:lang="deu">We applied exogenous guanosine trisphosphate, GTP, to Paramecium tetraurelia cells injected with Fura Red for analysing changes of free intracellular Ca2+ concentrations, [Ca2+]i, during periodic back-/forward swimming thus induced. Strain ginA (non-responsive to GTP) shows no Ca2+ signal upon GTP application. In strain nd6 (normal Ca2+ signalling) an oscillating [Ca2+]i response with a prominent first peak occurs upon GTP stimulation, but none after mock-stimulation or after 15 min adaptation to GTP. While this is in agreement with previous electrophysiological analyses, we now try to identify more clearly the source(s) of Ca2+. Stimulation of nd6 cells, after depletion of Ca2+ from their cortical stores (alveolar sacs), shows the same Ca2+ oscillation pattern but with reduced amplitudes, and a normal behavioural response is observed. Stimulation with GTP, supplemented with the Ca2+ chelator BAPTA, results in loss of the first prominent Ca2+ peak, in reduction of the following Ca2+ amplitudes, and in the absence of any behavioural response. Both these observations strongly suggest that for the initiation of GTP-mediated back-/forward swimming Ca2+ from the extracellular medium is needed. For the maintenance of the Ca2+ oscillations a considerable fraction must come from internal stores, probably other than alveolar sacs, rather likely from the endoplasmic reticulum.</dcterms:abstract>
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    <dc:creator>Plattner, Helmut</dc:creator>
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    <dc:contributor>Plattner, Helmut</dc:contributor>
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