@article{SoaresMedeiros2005proto-7525,
  year={2005},
  doi={10.1016/j.molbiopara.2004.12.012},
  title={A proton pumping pyrophosphatase in acidocalcisomes of Herpetomonas sp.},
  number={2},
  volume={140},
  issn={0166-6851},
  journal={Molecular and Biochemical Parasitology},
  pages={175--182},
  author={Soares Medeiros, Lia Carolina A. and Moraes Moreira, Bernardo Luis and Miranda, Kildare and Souza, Wanderley de and Plattner, Helmut and Hentschel, Joachim and Barrabin, Hector}
}

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    <dc:contributor>Barrabin, Hector</dc:contributor>
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    <dc:rights>Attribution-NonCommercial-NoDerivs 2.0 Generic</dc:rights>
    <dc:contributor>Miranda, Kildare</dc:contributor>
    <dc:creator>Soares Medeiros, Lia Carolina A.</dc:creator>
    <dc:contributor>Hentschel, Joachim</dc:contributor>
    <dc:creator>Souza, Wanderley de</dc:creator>
    <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-24T17:35:07Z</dcterms:available>
    <dc:creator>Moraes Moreira, Bernardo Luis</dc:creator>
    <dc:language>eng</dc:language>
    <dc:contributor>Moraes Moreira, Bernardo Luis</dc:contributor>
    <dc:creator>Miranda, Kildare</dc:creator>
    <dc:contributor>Plattner, Helmut</dc:contributor>
    <dcterms:abstract xml:lang="eng">Acidocalcisomes are acidic calcium storage organelles found in several microorganisms. They are characterized by their acidic nature, high electron density, high content of polyphosphates and several cations. Electron microscopy contrast tuned images of Herpetomonas sp. showed the presence of several electron dense organelles ranging from 100 to 300 nm in size. In addition, X-ray element mapping associated with energy-filtering transmission electron microscopy showed that most of the cations, namely Na, Mg, P, K, Fe and Zn, are located in their matrix. Using acridine orange as an indicator dye, a pyrophosphate-driven H+ uptake was measured in cells permeabilized by digitonin. This uptake has an optimal pH of 6.5 6.7 and was inhibited by sodium fluoride (NaF) and imidodiphosphate (IDP), two H+-pyrophosphatase inhibitors. H+ uptake was not promoted by ATP. Addition of 50 μMCa2+ induced the release of H+, suggesting the presence of a Ca2+/H+ countertransport system in the membranes of the acidic compartments. Na+ was unable to release protons from the organelles. The pyrophosphate-dependent H+ uptake was dependent of ion K+ and inhibited by Na+ Herpetomonas sp. immunolabeled with monoclonal antibodies raised against a Trypanosoma cruzi V-H+-pyrophosphatase shows intense fluorescence in cytoplasmatic organelles of size and distribution similar to the electron-dense vacuoles. Together, these results suggest that the electron dense organelles found in Herpetomonas sp. are homologous to the acidocalcisomes described in other trypanosomatids. They possess a vacuolar H+-pyrophosphatase and a Ca2+/H+ antiport. However, in contrast to the other trypanosomatids so far studied, we were not able to measure any ATP promoted H+ transport in the acidocalcisomes of this parasite.</dcterms:abstract>
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    <dc:contributor>Souza, Wanderley de</dc:contributor>
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    <dc:creator>Plattner, Helmut</dc:creator>
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    <dc:creator>Hentschel, Joachim</dc:creator>
    <dc:contributor>Soares Medeiros, Lia Carolina A.</dc:contributor>
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    <dcterms:bibliographicCitation>First publ. in: Molecular and Biochemical Parasitology 140 (2005), pp. 175-182</dcterms:bibliographicCitation>
    <dcterms:title>A proton pumping pyrophosphatase in acidocalcisomes of Herpetomonas sp.</dcterms:title>
    <dc:creator>Barrabin, Hector</dc:creator>
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