The vacuolar proton-ATPase plays a major role in several membrane-bounded organelles in Paramecium

Wassmer, Thomas; Froissard, Marine; Plattner, Helmut; Kissmehl, Roland; Cohen, Jean

The vacuolar proton-ATPase plays a major role in several membrane-bounded organelles in Paramecium

Dateien

The_vacuolar_proton.pdfGröße: 1.29 MBDownloads: 278

Datum

2005

Autor:innen

Wassmer, Thomas

Froissard, Marine

Plattner, Helmut

Kissmehl, Roland

Cohen, Jean

URI (zitierfähiger Link)

http://nbn-resolving.de/urn:nbn:de:bsz:352-opus-42888

Link zur Lizenz

Attribution-NonCommercial-NoDerivs 2.0 Generic

Open Access-Veröffentlichung

Open Access Green

Sammlungen

Biologie

Publikationstyp

Zeitschriftenartikel

Publikationsstatus

Published

Erschienen in

Journal of Cell Science. 2005, 118(13), pp. 2813-2825. ISSN 0021-9533. eISSN 1477-9137

Zusammenfassung

The vacuolar proton-ATPase (V-ATPase) is a multisubunit enzyme complex that is able to transfer protons over membranes against an electrochemical potential under ATP hydrolysis. The enzyme consists of two subcomplexes: V0, which is membrane embedded; and V1, which is cytosolic. V0 was also reported to be involved in fusion of vacuoles in yeast. We identified six genes encoding csubunits (proteolipids) of V0 and two genes encoding Fsubunits of V1 and studied the role of the V-ATPase in trafficking in Paramecium. Green fluorescent protein (GFP) fusion proteins allowed a clear subcellular localization of c- and F-subunits in the contractile vacuole complex of the osmoregulatory system and in food vacuoles. Several other organelles were also detected, in particular dense core secretory granules (trichocysts). The functional significance of the V-ATPase in Paramecium was investigated by RNA interference (RNAi), using a recently developed feeding method. A novel strategy was used to block the expression of all six c- or both F-subunits simultaneously. The V-ATPase was found to be crucial for osmoregulation, the phagocytotic pathway and the biogenesis of dense core secretory granules. No evidence was found supporting participation of V0 in membrane fusion.

Fachgebiet (DDC)

570 Biowissenschaften, Biologie

Schlagwörter

V-ATPase, RNAi, Contractile vacuole, Phagosome, Trichocyst

Zitieren

ISO 690

WASSMER, Thomas, Marine FROISSARD, Helmut PLATTNER, Roland KISSMEHL, Jean COHEN, 2005. The vacuolar proton-ATPase plays a major role in several membrane-bounded organelles in Paramecium. In: Journal of Cell Science. 2005, 118(13), pp. 2813-2825. ISSN 0021-9533. eISSN 1477-9137

BibTex

@article{Wassmer2005vacuo-7009,
  year={2005},
  title={The vacuolar proton-ATPase plays a major role in several membrane-bounded organelles in Paramecium},
  number={13},
  volume={118},
  issn={0021-9533},
  journal={Journal of Cell Science},
  pages={2813--2825},
  author={Wassmer, Thomas and Froissard, Marine and Plattner, Helmut and Kissmehl, Roland and Cohen, Jean}
}

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Universitätsbibliographie

Ja