@article{Murray1997Mecha-9726,
  year={1997},
  doi={10.1021/bi962284z},
  title={Mechanism of human alpha-1,3-fucosyltransferase V : glycosidic cleavage occurs prior to nucleophilic attack},
  number={4},
  volume={36},
  issn={0006-2960},
  journal={Biochemistry},
  pages={823--831},
  author={Murray, Brion W. and Wittmann, Valentin and Burkart, Michael D. and Hung, Shang-Cheng and Wong, Chi-Huey}
}

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    <dc:creator>Burkart, Michael D.</dc:creator>
    <dc:contributor>Wong, Chi-Huey</dc:contributor>
    <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-24T18:13:58Z</dc:date>
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    <dc:contributor>Hung, Shang-Cheng</dc:contributor>
    <dcterms:issued>1997</dcterms:issued>
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    <dc:creator>Hung, Shang-Cheng</dc:creator>
    <dc:contributor>Murray, Brion W.</dc:contributor>
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    <dcterms:bibliographicCitation>First publ. in: Biochemistry ; 36 (1997). - S. 823-831</dcterms:bibliographicCitation>
    <dcterms:abstract xml:lang="eng">α-1,3-Fucosyltransferase V (FucT V) catalyzes the transfer of l-fucose from the donor sugar guanosine 5'-diphospho-β-l-fucose (GDP-Fuc) to an acceptor sugar. A secondary isotope effect on the fucosyltransfer reaction with guanosine 5'-diphospho-[1-²H]-β-l-fucose (GDP-[1-²H]-Fuc) as the substrate was observed and determined to be DV = 1.32 + 0.13 and DV/K = 1.27 + 0.07. Competitive inhibition of FucT V by guanosine 5'-diphospho-2-deoxy-2-fluoro-β-l-fucose (GDP-2F-Fuc) was observed with an inhibition constant of 4.2 íM which represents the most potent inhibitor of this enzyme to date. Incubation of GDP-2F-Fuc with FucT V and an acceptor molecule prior to the addition of GDP-Fuc had no effect on the potency of inhibition, indicating that GDP-2F-Fuc is neither an inactivator nor a slow substrate. Both the observed secondary isotope effect and the inhibition by GDP-2F-Fuc are consistent with a charged, sp²-hybridized, transition-state structure. A convenient and efficient synthesis of GDP-[1-²H]-Fuc and GDP-2F-Fuc and a nonradioactive, fluorescence assay for fucosyltransferase activity have been developed.</dcterms:abstract>
    <dc:creator>Wong, Chi-Huey</dc:creator>
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    <dc:contributor>Wittmann, Valentin</dc:contributor>
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    <dcterms:title>Mechanism of human alpha-1,3-fucosyltransferase V : glycosidic cleavage occurs prior to nucleophilic attack</dcterms:title>
    <dc:contributor>Burkart, Michael D.</dc:contributor>
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