Chitinases and beta-1,3-Glucanases in the Apoplastic Compartment of Oat Leaves (Avena sativa L.)

Fink, Werner; Liefland, Mathias; Mendgen, Kurt

Chitinases and beta-1,3-Glucanases in the Apoplastic Compartment of Oat Leaves (Avena sativa L.)

Dateien

Chitinases_and_beta_13_Glucanases_in_the_Apoplastic_Compartment_of_Oat_Leaves.pdfGröße: 1.27 MBDownloads: 279

Datum

1988

Autor:innen

Fink, Werner

Liefland, Mathias

Mendgen, Kurt

Open Access-Veröffentlichung

Open Access Green

Sammlungen

Biologie

Publikationstyp

Zeitschriftenartikel

Publikationsstatus

Published

Erschienen in

Plant Physiology. 1988, 88(2), pp. 270-275. ISSN 0032-0889. eISSN 1532-2548. Available under: doi: 10.1104/pp.88.2.270

Zusammenfassung

To isolate chitinases and beta-1,3-glucanases from the intercellular space of oats (Avena sativa L.), primary leaves were infiltrated with buffer and subjected to gentle centrifugation to obtain intercellular washing fluid (IWF). Approximately 5% of the chitinase and 10% of the beta-1,3-glucanase activity of the whole leaf were released. Only small amounts (0.01-0.03%) of the intracellular marker malate-dehydrogenase were released into the IWF during infiltration. Activities of chitinase and beta-1,3-glucanase in the IWF and in the leaf extract were compared by different chromatographic methods. On Sephadex G-75, chitinase appeared as a single peak (M, 29.8 kD) both in IWF and homogenate. Beta-1,3-Glucanase, however, showed two peaks in the IWF (M, 52 and 31.3 kD), whereas the elution pattern of the homogenate showed only one major peak at 22 kD. Chromatofocusing indicated that the IWF contained four chitinases and five beta-1,3-glucanases. The elution pattern of the homogenate and IWF were similar with regard to the elution pH, but the peak intensities were distinctly different. Our results demonstrate that extracellular beta-1,3-glucanases are different from those located intracellularly. Extracellular and intracellular chitinases do not differ in molecular properties, except for one isozyme which seems to be confined to the extracellular space. We suggest that both enzymes might play a special role in pathogenesis during fungal infection.

Fachgebiet (DDC)

570 Biowissenschaften, Biologie

Zitieren

ISO 690

FINK, Werner, Mathias LIEFLAND, Kurt MENDGEN, 1988. Chitinases and beta-1,3-Glucanases in the Apoplastic Compartment of Oat Leaves (Avena sativa L.). In: Plant Physiology. 1988, 88(2), pp. 270-275. ISSN 0032-0889. eISSN 1532-2548. Available under: doi: 10.1104/pp.88.2.270

BibTex

@article{Fink1988Chiti-7990,
  year={1988},
  doi={10.1104/pp.88.2.270},
  title={Chitinases and beta-1,3-Glucanases in the Apoplastic Compartment of Oat Leaves (Avena sativa L.)},
  number={2},
  volume={88},
  issn={0032-0889},
  journal={Plant Physiology},
  pages={270--275},
  author={Fink, Werner and Liefland, Mathias and Mendgen, Kurt}
}

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Universitätsbibliographie

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