Identification of astrocyte- and oligodendrocyte-like cells of goldfish optic nerves in culture

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1989
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Bastmeyer, Martin
Beckmann, Matthias
Nona, Shmaiel N.
Cronly-Dillon, John R.
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Neuroscience Letters. 1989, 101(2), pp. 127-132. ISSN 0304-3940. Available under: doi: 10.1016/0304-3940(89)90518-1
Zusammenfassung

Fish glial cells were obtained from cultivated segments of the optic nerve and raised in vitro. Two types of cells were identified as astrocyte- and oligodendrocyte-like glia by the monoclonal antibody Mab O1 (specific for oligodendrocytes) and the rabbit serum anti-goldfish glial fibrillary acidic protein (anti-G-GFAP). Cells of compact morphology were rare, and anti-G-GFAP positive and O1 negative. Multipolar cells in 5-day-old cultures were anti-G-GFAP but rarely O1 positive. In 5-week-old cultures, however, roughly 75% of the multipolar cells were double-labeled with both anti-G-GFAP and O1; 10% were single labeled with Mab O1 and 15% with anti-G-GFAP, respectively.

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Fachgebiet (DDC)
570 Biowissenschaften, Biologie
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Glial cell, Goldfish optic nerve, In culture, Immunostaining
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ISO 690BASTMEYER, Martin, Matthias BECKMANN, Shmaiel N. NONA, John R. CRONLY-DILLON, Claudia STÃœRMER, 1989. Identification of astrocyte- and oligodendrocyte-like cells of goldfish optic nerves in culture. In: Neuroscience Letters. 1989, 101(2), pp. 127-132. ISSN 0304-3940. Available under: doi: 10.1016/0304-3940(89)90518-1
BibTex
@article{Bastmeyer1989Ident-6629,
  year={1989},
  doi={10.1016/0304-3940(89)90518-1},
  title={Identification of astrocyte- and oligodendrocyte-like cells of goldfish optic nerves in culture},
  number={2},
  volume={101},
  issn={0304-3940},
  journal={Neuroscience Letters},
  pages={127--132},
  author={Bastmeyer, Martin and Beckmann, Matthias and Nona, Shmaiel N. and Cronly-Dillon, John R. and Stürmer, Claudia}
}
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