@article{Koster1996Locat-8396,
  year={1996},
  doi={10.1111/j.1432-1033.1996.00858.x},
  title={Location of the Active Site and Proposed Catalytic Mechanism of Pterin-4A-Carbinolamine Dehydratase},
  number={3},
  volume={241},
  issn={0014-2956},
  journal={European Journal of Biochemistry},
  pages={858--864},
  author={Köster, Sandra and Stier, Gunter and Ficner, Ralf and Hölzer, Manuela and Curtius, Hans-Christoph and Suck, Dietrich and Ghisla, Sandro}
}

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    <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-24T17:43:16Z</dc:date>
    <dc:contributor>Köster, Sandra</dc:contributor>
    <dc:creator>Hölzer, Manuela</dc:creator>
    <dc:contributor>Suck, Dietrich</dc:contributor>
    <dc:contributor>Ghisla, Sandro</dc:contributor>
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    <dc:language>eng</dc:language>
    <dc:contributor>Hölzer, Manuela</dc:contributor>
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    <dc:creator>Suck, Dietrich</dc:creator>
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    <dc:contributor>Ficner, Ralf</dc:contributor>
    <dc:contributor>Curtius, Hans-Christoph</dc:contributor>
    <dcterms:bibliographicCitation>First publ. in: European Journal of Biochemistry 241 (1996), 3, pp. 858-864</dcterms:bibliographicCitation>
    <dcterms:abstract xml:lang="eng">Based on the recently solved three-dimensional structure of pterin-4a-carbinolamine dehydratase from rat/human liver the involvement of the proposed active-site residues Glu57, Asp60, His61, His62, Tyr69, His79, Arg87 and Asp88 was examined by site-directed mutagenesis. Most of the mutants showed reduced activity, and only the Glu57→Ala mutant and the His61→Ala, His62→Ala double mutant were fully devoid of activity. The dissociation constants of quinonoid 6,6-dimethyl-7,8-dihydropterin were significantly increased for binding to the Glu57→Ala, His61→Ala, His62→Ala single mutants and the His61→Ala, His62→Ala double mutant, confirming that His61 and His62 are essential for substrate binding and catalysis. The mechanism of dehydration is proposed to involve base catalysis at the N(5)-H group of the substrate by His61.</dcterms:abstract>
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    <dcterms:title>Location of the Active Site and Proposed Catalytic Mechanism of Pterin-4A-Carbinolamine Dehydratase</dcterms:title>
    <dcterms:issued>1996</dcterms:issued>
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    <dc:contributor>Stier, Gunter</dc:contributor>
    <dc:creator>Köster, Sandra</dc:creator>
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    <dc:creator>Ficner, Ralf</dc:creator>
    <dc:creator>Curtius, Hans-Christoph</dc:creator>
    <dc:creator>Stier, Gunter</dc:creator>
    <dc:creator>Ghisla, Sandro</dc:creator>
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