@article{Steinerstauch1989Purif-8583,
  year={1989},
  doi={10.1515/pteridines.1989.1.4.189},
  title={Purification and characterization of a carbonyl-reductase from human liver, which is competent in the reduction of 6-pyruvoyl-tetrahydropterin},
  number={4},
  volume={1},
  issn={0933-4807},
  journal={Pteridines},
  pages={189--198},
  author={Steinerstauch, Petra and Sawada, Yoshitomo and Leimbacher, Walter and Ghisla, Sandro and Curtius, Hans-Christoph}
}

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    <dc:creator>Sawada, Yoshitomo</dc:creator>
    <dc:contributor>Ghisla, Sandro</dc:contributor>
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    <dcterms:issued>1989</dcterms:issued>
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    <dc:language>eng</dc:language>
    <dc:creator>Curtius, Hans-Christoph</dc:creator>
    <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-24T17:44:52Z</dc:date>
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    <dcterms:abstract xml:lang="eng">An enzyme which reduces 6-pyruvoyl-tetrahydropterin has been purified to apparent homogeneity from human liver. It consists of a single polypeptide chain with a molecular weight of 35 kDa, has an isoelectric point of 5.9 ± 0.1 and contains no glycosyl residues. The pure enzyme has a specific activity of 450 mU/mg protein at pH 7.0 in 10 mM potassium phosphate buffer. It converts 6-pyruvoyl-tetrahydropterin to 6-lactoyltetrahydropterin by transfer of the pro 4R-hydrogen of NADPH to form the side chain -OH at position C(2') of the substrate. Km values are 1.8 μM for 6-pyruvoyl-tetrahydropterin and 5.5 μM for NADPH. Polyclonal antibodies raised against the purified enzyme recognize 6-pyruvoyl-tetrahydropterin reductase in Western blot and ELISA but do not cross-react with human sepiapterin reductase. The enzyme appears to be identical with aldose reductase.</dcterms:abstract>
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    <dc:contributor>Steinerstauch, Petra</dc:contributor>
    <dc:contributor>Sawada, Yoshitomo</dc:contributor>
    <dc:contributor>Leimbacher, Walter</dc:contributor>
    <dcterms:title>Purification and characterization of a carbonyl-reductase from human liver, which is competent in the reduction of 6-pyruvoyl-tetrahydropterin</dcterms:title>
    <dc:rights>Attribution-NonCommercial-NoDerivs 2.0 Generic</dc:rights>
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    <dcterms:bibliographicCitation>First publ. in: Pteridines 1 (1989), 4, pp. 189-198</dcterms:bibliographicCitation>
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    <dc:creator>Leimbacher, Walter</dc:creator>
    <dc:creator>Steinerstauch, Petra</dc:creator>
    <dc:contributor>Curtius, Hans-Christoph</dc:contributor>
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