Probing multivalent carbohydrate-lectin interactions by an enzyme-linked lectin assay employing covalently immobilized carbohydrates

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2007
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Maierhofer, Caroline
Rohmer, Katja
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Bioorganic & Medicinal Chemistry. 2007, 15(24), pp. 7661-7676. ISSN 0968-0896. eISSN 1464-3391. Available under: doi: 10.1016/j.bmc.2007.08.063
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We report here the synthesis of a series of mono- to trivalent N-acetylglucosamine (GlcNAc) derivatives as ligands for the plant lectin wheat germ agglutinin (WGA). Their WGA binding potencies were determined by an established enzyme-linked lectin assay (ELLA) employing microtiter plates with non-covalently immobilized porcine stomach mucin (PSM) as reference ligand and an ELLA with a new GlcNAc derivative covalently immobilized via a thiourea linkage. Comparison of both assays revealed that the type of presentation of GlcNAc residues on the microtiter plates either as part of a glycoprotein or as a covalently immobilized monosaccharide derivative strongly influences the outcome of the assay. Although the apparent dissociation constants K/ELLA/D for the interaction of peroxidase-labeled WGA with the microtiter plates are comparable for both surfaces, IC50 values obtained with the PSM-free ELLA were substantially lower. Even more strikingly, this ELLA displayed a better differentiation between ligands of different valency leading to significantly higher relative inhibitory potencies of multivalent ligands compared to monovalent. Additionally, problems associated with the use of PSM, such as maximum inhibition at considerably less than 100% and poor reproducibility of IC50 values could be overcome with this type of ELLA.

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ISO 690MAIERHOFER, Caroline, Katja ROHMER, Valentin WITTMANN, 2007. Probing multivalent carbohydrate-lectin interactions by an enzyme-linked lectin assay employing covalently immobilized carbohydrates. In: Bioorganic & Medicinal Chemistry. 2007, 15(24), pp. 7661-7676. ISSN 0968-0896. eISSN 1464-3391. Available under: doi: 10.1016/j.bmc.2007.08.063
BibTex
@article{Maierhofer2007Probi-9830,
  year={2007},
  doi={10.1016/j.bmc.2007.08.063},
  title={Probing multivalent carbohydrate-lectin interactions by an enzyme-linked lectin assay employing covalently immobilized carbohydrates},
  number={24},
  volume={15},
  issn={0968-0896},
  journal={Bioorganic & Medicinal Chemistry},
  pages={7661--7676},
  author={Maierhofer, Caroline and Rohmer, Katja and Wittmann, Valentin}
}
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